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首页> 外文期刊>Environmental Science & Technology >Comparison of Cytotoxicity and Inhibition of Membrane ABC Transporters Induced by MWCNTs with Different Length and Functional Groups
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Comparison of Cytotoxicity and Inhibition of Membrane ABC Transporters Induced by MWCNTs with Different Length and Functional Groups

机译：不同长度和官能团的MWCNTs诱导的细胞毒性和膜ABC转运蛋白的抑制作用比较

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Experimental studies indicate that multiwalled carbon nanotubes (MWCNTs) have the potential to induce cytotoxicity. However, the reports are often inconsistent and even contradictory. Additionally, adverse effects of MWCNTs at low concentration are not well understood. In this study, we systemically compared adverse effects of six MWCNTs including pristine MWCNTs, hydroxyl-MWCNTs and carboxyl-MWCNTs of two different lengths (0.5-2 μm and 10-30 μm) on human hepatoma cell line HepG2. Results showed that MWCNTs induced cytotoxicity by increasing reactive oxygen species (ROS) generation and damaging cell function. Pristine short MWCNTs induced higher cytotoxicity than pristine long MWCNTs. Functionalization increased cytotoxicity of long MWCNTs, but reduced cytotoxicity of short MWCNTs. Further, our results indicated that the six MWCNTs, at nontoxic concentration, might not be environmentally safe as they inhibited ABC transporters' efflux capabilities. This inhibition was observed even at very low concentrations, which were 40- 1000 times lower than their effective concentrations on cytotoxicity. The inhibition of ABC transporters significantly increased cytotoxicity of arsenic a known substrate of ABC transporters, indicating a chemosensitizing effect of MWCNTs. Plasma membrane damage was likely the mechanism by which the six MWCNTs inhibited ABC transporter activity. This study provides insight into risk assessments of low levels of MWCNTs in the environment.
机译：实验研究表明，多壁碳纳米管（MWCNTs）具有诱导细胞毒性的潜力。但是，这些报告常常前后矛盾，甚至相互矛盾。另外，人们还不太了解低浓度的MWCNT的不利影响。在这项研究中，我们系统地比较了两种不同长度（0.5-2μm和10-30μm）的原始MWCNT，羟基MWCNT和羧基MWCNT对六种MWCNT对人肝癌细胞HepG2的不利影响。结果表明，MWCNTs通过增加活性氧（ROS）的生成和破坏细胞功能来诱导细胞毒性。原始的短MWCNT诱导的细胞毒性高于原始的长MWCNT。功能化增加了长MWCNT的细胞毒性，但降低了短MWCNT的细胞毒性。此外，我们的结果表明，六个无毒浓度的MWCNTs可能会抑制ABC转运蛋白的外排能力，因此可能对环境不安全。即使在非常低的浓度下也观察到这种抑制作用，该浓度比其对细胞毒性的有效浓度低40-1000倍。 ABC转运蛋白的抑制作用显着增加了已知的ABC转运蛋白底物砷的细胞毒性，表明MWCNT的化学增敏作用。质膜损伤可能是六个MWCNT抑制ABC转运蛋白活性的机制。这项研究提供了对环境中低水平碳纳米管风险评估的见识。

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  • 来源
    《Environmental Science & Technology》 |2016年第7期|3985-3994|共10页
  • 作者

    Jing Yu; Su Liu; Bing Wu; Zhuoyan Shen; Gary N. Cherr; Xu-Xiang Zhang; Mei Li;

  • 作者单位

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China;

    Bodega Marine Laboratory, University of California, Davis, California United States,Departments of Environmental Toxicology and Nutrition, University of California, Davis, California United States;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, P.R. China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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