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首页> 外文期刊>Environmental Science & Technology >Combined Transcriptomic and Proteomic Approach to Identify Toxicity Pathways in Early Life Stages of Japanese Medaka (Oryzias latipes) Exposed to 1,2,5,6-Tetrabromocyclooctane (TBCO)
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Combined Transcriptomic and Proteomic Approach to Identify Toxicity Pathways in Early Life Stages of Japanese Medaka (Oryzias latipes) Exposed to 1,2,5,6-Tetrabromocyclooctane (TBCO)

机译:转录组和蛋白质组学方法识别暴露于1,2,5,6-四溴环辛烷(TBCO)的日本Medaka(Oryzias latipes)生命早期的毒性途径

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摘要

Currently, the novel brominated flame retard-ant 1,2,5,6-tetrabromocyclooctane (TBCO) is considered a potential replacement for hexabromocyclododecane (HBCD). Therefore, use of TBCO could increase in the near future. To assess potential toxicological risks to aquatic organisms, embryos of Japanese medaka (Oryzias latipes) were exposed to 10, 100, or 1000 μg/L TBCO from 2 h postfertilization until 1 day post-hatch. TBCO accumulated in embryos in the order of 0.43-1.3 × 10~4-fold, and the rate constant of accumulation was 1.7-1.8 per day. The number of days to hatch and the hatching success of embryos exposed to the medium and the greatest concentrations of TBCO were impaired. Responses of the transcriptome (RNA-seq) and proteome were characterized in embryos exposed to 100μg/L TBCO because this was the least concentration of TBCO that caused an effect on hatching. Consistent with effects on hatching, proteins whose abundances were reduced by exposure to TBCO were enriched in embryo development and hatching pathways. Also, on the basis of the responses of transcriptome and proteome, it was predicted that TBCO might impair vision and contraction of cardiac muscle, respectively, and these effects were confirmed by targeted bioassays. This study provided a comprehensive understanding of effects of TBCO on medaka at early life stages and illustrated the power of "omics" to explain and predict phenotypic responses to chemicals.
机译:目前,新型溴化阻燃剂1,2,5,6-四溴环辛烷(TBCO)被认为是六溴环十二烷(HBCD)的潜在替代品。因此,在不久的将来TBCO的使用可能会增加。为了评估对水生生物的潜在毒理学风险,从受精后2小时到孵化后1天,将日本(Oryzias latipes)的胚暴露于10、100或1000μg/ L TBCO。 TBCO在胚胎中的积累量为0.43-1.3×10〜4倍,每天的积累速率常数为1.7-1.8。暴露于培养基和最大浓度的TBCO的胚胎的孵化天数和孵化成功受到损害。在暴露于100μg/ L TBCO的胚胎中表征了转录组(RNA-seq)和蛋白质组的反应,因为这是导致孵化效果的最低TBCO浓度。与对孵化的影响一致,通过暴露于TBCO可以降低丰度的蛋白质富含胚胎发育和孵化途径。此外,根据转录组和蛋白质组的反应,可以预料TBCO可能分别损害视力和心肌收缩,这些作用已通过靶向生物测定法得到证实。这项研究提供了对TBCO在生命早期对medaka的影响的全面理解,并阐明了“组学”解释和预测对化学物质的表型反应的能力。

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  • 来源
    《Environmental Science & Technology》 |2016年第14期|7781-7790|共10页
  • 作者单位

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada;

    School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C8, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada,School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C8, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada,School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C8, Canada;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada,Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada,Zoology Department, Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan 48824, United States,School of Biological Sciences, University of Hong Kong, Hong Kong Special Administrative Region 999077, People's Republic of China,State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, People's Republic of China;

    Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada,Department of Biology, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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