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首页> 外文期刊>Environmental Science & Technology >Metabolomics to Detect Response of Lettuce (Lactuca sativa) to Cu(OH)_2 Nanopesticides: Oxidative Stress Response and Detoxification Mechanisms
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Metabolomics to Detect Response of Lettuce (Lactuca sativa) to Cu(OH)_2 Nanopesticides: Oxidative Stress Response and Detoxification Mechanisms

机译:代谢组学检测莴苣(Lactuca sativa)对Cu(OH)_2纳米农药的响应:氧化应激响应和解毒机理

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摘要

There has been an increasing influx of nanopesticides into agriculture in recent years. Understanding the interaction between nanopesticides and edible plants is crucial in evaluating the potential impact of nanotechnology on the environment and agriculture. Here we exposed lettuce plants to Cu(OH)_2 nanopesticides (1050-2100 mg/L) through foliar spray for one month. Inductively coupled plasma-mass spectrometry (ICP-MS) results indicate that 97-99% (1353-2501 mg/ kg) of copper was sequestered in the leaves and only a small percentage (1-3%) (17.5-56.9 mg/kg) was translocated to root tissues through phloem loading. Gas chromatography-time-of- flight mass spectrometry (GC-TOF-MS) based metabolomics combined with partial least squares-discriminant analysis (PLS-DA) multivariate analysis revealed that Cu(OH)_2 nanopesticides altered metabolite levels of lettuce leaves. Tricarboxylic (TCA) cycle and a number of amino acid-related biological pathways were disturbed. Some antioxidant levels (cis-caffeic acid, chlorogenic acid, 3,4-dihydroxycinnamic acid, dehydroascorbic acid) were significantly decreased compared to the control, indicating that oxidative stress and a defense response occurred. Nicotianamine, a copper chelator, increased by 12-27 fold compared to the control, which may represent a detoxification mechanism. The up-regulation of polyamines (spermidine and putrescine) and potassium may mitigate oxidative stress and enhance tolerance. The data presented here provide a molecular-scale perspective on the response of plants to copper nanopesticides.
机译:近年来,越来越多的纳米农药进入农业。了解纳米农药与食用植物之间的相互作用对于评估纳米技术对环境和农业的潜在影响至关重要。在这里,我们通过叶面喷洒将生菜植物暴露于Cu(OH)_2纳米杀虫剂(1050-2100 mg / L)下一个月。电感耦合等离子体质谱法(ICP-MS)结果表明,叶片中螯合了97-99%(1353-2501 mg / kg)的铜,只有一小部分(1-3%)(17.5-56.9 mg / kg)通过韧皮部加载将其转移到根组织。基于气相色谱-飞行时间质谱(GC-TOF-MS)的代谢组学与偏最小二乘判别分析(PLS-DA)多变量分析相结合,揭示了Cu(OH)_2纳米农药改变了莴苣叶片的代谢产物水平。三羧酸(TCA)循环和许多氨基酸相关的生物途径受到干扰。与对照相比,一些抗氧化剂的水平(顺式咖啡酸,绿原酸,3,4-二羟基肉桂酸,脱氢抗坏血酸)显着降低,表明发生了氧化应激和防御反应。烟碱胺(一种铜螯合剂)与对照相比增加了12-27倍,这可能代表一种排毒机制。多胺(亚精胺和腐胺)和钾的上调可减轻氧化应激并增强耐受性。此处提供的数据为植物对铜纳米农药的反应提供了分子尺度的视角。

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  • 来源
    《Environmental Science & Technology》 |2016年第17期|9697-9707|共11页
  • 作者单位

    Bren School of Environmental Science & Management, University of California, Santa Barbara, California 93106-5131, United States,University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, California United States;

    Bren School of Environmental Science & Management, University of California, Santa Barbara, California 93106-5131, United States,University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, California United States;

    Bren School of Environmental Science & Management, University of California, Santa Barbara, California 93106-5131, United States,University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, California United States;

    Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, California 93106, United States;

    Neurosrience Research Institute and Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California 93106, United States;

    Bren School of Environmental Science & Management, University of California, Santa Barbara, California 93106-5131, United States,University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, California United States;

    Bren School of Environmental Science & Management, University of California, Santa Barbara, California 93106-5131, United States,University of California, Center for Environmental Implications of Nanotechnology, Santa Barbara, California United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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