...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >In Vivo Bioavailability and In Vitro Bioaccessibility of Perfluorooctanoic Acid (PFOA) in Food Matrices: Correlation Analysis and Method Development
【24h】

In Vivo Bioavailability and In Vitro Bioaccessibility of Perfluorooctanoic Acid (PFOA) in Food Matrices: Correlation Analysis and Method Development

机译：食品基质中全氟辛酸（PFOA）的体内生物利用度和体外生物利用度：相关性分析和方法开发

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Food is a major source of human exposure to perfluorooctanoic acid (PFOA), however, PFOA bioavailability in food has not been studied. An in vivo mouse model and three in vitro methods (unified BARGE method, UBM; physiologically based extraction test, PBET; and in vitro digestion method, IVD) were used to determine the relative bioavailability and bioaccessibility of PFOA in the presence of 17 foods. PFOA was mixed with foods of different nutritional compositions and fed to mice over a 7-d period. PFOA relative bioavailability was determined by comparing PFOA accumulation in the liver following PFOA exposure via food to that in water. PFOA bioavailability relative to water ranged from 4.30 ± 0.80 to 69.0 ± 11.9% and was negatively correlated with lipid content (r = 0.76). This was possibly due to competitive sorption of free fatty acids with PFOA onto transporters on intestine epithelial cells. Besides, cations in the gastrointestinal tract, such as Ca~(2+) and Mg~(2+), are capable of complexing PFOA and partitioning to the lipid phase. On the other hand, when assessed using in vitro assays, PFOA bioaccessibility varied with methods, being 8.7-73% (UBM), 9.8-9996 (PBET), and 21-114% (IVD). PFOA bioaccessibility was negatively correlated with lipid content when assessed using UBM (r = 0.82); however, a poor correlation with food composition was observed for PBET and IVD (r = 0.01-0.50). When in vivo and in vitro data were compared, a strong correlation was observed for UBM (r = 0.79), but poor relationships were observed for PBET and IVD (r = 0.11-0.22). This was probably because the higher lipolysis ability and presence of Ca~(2+) and Mg~(2+) in the gastrointestinal fluid of UBM resulted in a lower potential to form stable micelles compared to PBET and IVD. These results indicated that PFOA relative bioavailability was mainly affected by lipid content in foods, and UBM has the potential to determine PFOA bioaccessibility in food samples.
机译：食物是人类暴露于全氟辛酸（PFOA）的主要来源，但是，尚未研究食物中PFOA的生物利用度。使用体内小鼠模型和三种体外方法（统一的BARGE方法，UBM；基于生理的提取试验，PBET；和体外消化方法，IVD）确定存在17种食物时PFOA的相对生物利用度和生物利用度。将PFOA与不同营养成分的食物混合，并在7天的时间内喂给小鼠。 PFOA相对生物利用度是通过比较PFOA通过食物与水中接触后在肝脏中的PFOA积累来确定的。相对于水，PFOA的生物利用度为4.30±0.80至69.0±11.9％，并且与脂质含量呈负相关（r = 0.76）。这可能是由于游离脂肪酸与PFOA在肠上皮细胞转运蛋白上的竞争性吸附。此外，胃肠道中的阳离子，例如Ca〜（2+）和Mg〜（2+），能够使PFOA络合并分配到脂质相。另一方面，当使用体外测定进行评估时，PFOA的生物可及性随方法而异，分别为8.7-73％（UBM），9.8-9996（PBET）和21-114％（IVD）。当使用UBM评估时，PFOA的生物可及性与脂质含量呈负相关（r = 0.82）；然而，对于PBET和IVD，与食品成分的相关性较差（r = 0.01-0.50）。比较体内和体外数据时，观察到UBM的相关性很强（r = 0.79），而观察到PBET和IVD的相关性很差（r = 0.11-0.22）。这可能是因为与PBET和IVD相比，UBM的胃肠液中较高的脂解能力和Ca〜（2+）和Mg〜（2+）的存在导致形成稳定胶束的可能性较低。这些结果表明，PFOA的相对生物利用度主要受食品中脂质含量的影响，而UBM具有确定食品样品中PFOA的生物利用度的潜力。

著录项

  • 来源
    《Environmental Science & Technology》 |2015年第1期|150-158|共9页
  • 作者

    Kan Li; Chao Li; Nan-Yang Yu; Albert L. Juhasz; Xin-Yi Cui; Lena Q. Ma;

  • 作者单位

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210046, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210046, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210046, China;

    Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, SA 5095, Australia;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210046, China;

    State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210046, China,Soil and Water Science Department, University of Florida, Gainesville, Florida 32611, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号