...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Noncovalent Binding of Polycyclic Aromatic Hydrocarbons with Genetic Bases Reducing the in Vitro Lateral Transfer of Antibiotic Resistant Genes
【24h】

Noncovalent Binding of Polycyclic Aromatic Hydrocarbons with Genetic Bases Reducing the in Vitro Lateral Transfer of Antibiotic Resistant Genes

机译：多环芳烃与遗传碱基的非共价结合减少了抗生素抗性基因的体外横向转移

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In current studies of noncovalent interactions of porycyclic aromatic hydrocarbons (PAHs) with genetic units, the impact of such interactions on gene transfer has not been explored. In this study, we examined the association of some widely occurring PAHs (phenanthrene, pyrene, benzo[g,h,i]perylene, and other congeners) with antibiotic resistant plasmids (pUC19). Small molecular PAHs (e.g., phenanthrene) bind effectively with plasmids to form a loosely clew-like plasmid-PAH complex (16.5-49.5 nm), resulting in reduced transformation of ampicillin resistance gene (Ampr). The in vitro transcription analysis demonstrated that reduced transformation of Ampr in plasmids results from the PAH-inhibited Ampr transcription to RNA. Fluorescence microtitration coupled with Fourier transform infrared spectroscopy (FTIR) and theoretical interaction models showed that adenine in plasmid has a stronger capacity to sequester small Phen and Pyre molecules via a π-π attraction. Changes in Gibbs free energy (ΔG) suggest that the CT-PAH model reliably depicts the plasmid-PAH interaction through a noncovalently physical sorption mechanism. Considering the wide occurrence of PAHs and antibiotic resistant genes (ARGs) in the environment, our findings suggest that small-sized PAHs can well affect the behavior of ARGs via above-described noncovalent interactions.
机译：在当前的对环芳香烃（PAHs）与遗传单位的非共价相互作用的研究中，尚未研究这种相互作用对基因转移的影响。在这项研究中，我们检查了一些广泛存在的PAH（菲，pyr，苯并[g，h，i] per和其他同类物）与抗生素抗性质粒（pUC19）的关联。小分子PAH（例如菲）与质粒有效结合，形成松散的线团状质粒-PAH复合物（16.5-49.5 nm），导致氨苄青霉素抗性基因（Ampr）的转化减少。体外转录分析表明，质粒中Ampr的转化减少归因于PAH抑制的Ampr转录为RNA。荧光微滴定结合傅里叶变换红外光谱（FTIR）和理论相互作用模型表明，质粒中的腺嘌呤具有更强的通过π-π螯合小分子Phen和Pyre分子的能力。吉布斯自由能（ΔG）的变化表明CT-PAH模型通过非共价物理吸附机制可靠地描述了质粒-PAH相互作用。考虑到环境中PAH和抗生素抗性基因（ARG）的广泛存在，我们的发现表明，小型PAH可以通过上述非共价相互作用很好地影响ARG的行为。

著录项

  • 来源
    《Environmental Science & Technology》 |2015年第17期|10340-10348|共9页
  • 作者

    Fuxing Kang; Xiaojie Hu; Juan Liu; Yanzheng Gao;

  • 作者单位

    Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;

    Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;

    Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;

    Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号