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首页> 外文期刊>Environmental Science & Technology >Characterization of Electrical Current-Generation Capabilities from Thermophilic Bacterium Thermoanaerobacter pseudethanolicus Using Xylose, Glucose, Cellobiose, or Acetate with Fixed Anode Potentials
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Characterization of Electrical Current-Generation Capabilities from Thermophilic Bacterium Thermoanaerobacter pseudethanolicus Using Xylose, Glucose, Cellobiose, or Acetate with Fixed Anode Potentials

机译:使用木糖,葡萄糖,纤维二糖或乙酸盐固定阳极电位,从嗜热嗜热嗜热细菌嗜热细菌中表征电流产生能力

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摘要

Thermoanaerobacter pseudethanolicus 39E (ATCC 33223), a thermophilic, Fe(Ⅲ)-reducing, and fermentative bacterium, was evaluated for its ability to produce current from four electron donors-xylose, glucose, cellobiose, and acetate-with a fixed anode potential (+ 0.042 V vs SHE) in a microbial electrochemical cell (MXC). Under thermophilic conditions (60 ℃), T. pseudethanolicus produced high current densities from xylose (5.8 ± 2.4 A m~(-2)), glucose (4.3 ±1.9 A m~(-2)), and cellobiose (5.2 ± 1.6 A m~(-2)). It produced insignificant current when grown with acetate, but consumed the acetate produced from sugar fermentation to produce electrical current. Low-scan cyclic voltammetry (LSCV) revealed a sigmoidal response with a midpoint potential of -0.17 V vs SHE. Coulombic efficiency (CE) varied by electron donor, with xylose at 34.8% ± 0.7%, glucose at 65.3% ± 1.0%, and cellobiose at 27.7% ± 1.5%. Anode respiration was sustained over a pH range of 5.4-8.3, with higher current densities observed at higher pH values. Scanning electron microscopy showed a well-developed biofilm of T. pseudethanolicus on the anode, and confocal laser scanning microscopy demonstrated a maximum biofilm thickness (L_f) greater than ~150 μm for the glucose-fed biofilm.
机译:对嗜热厌氧嗜热厌氧细菌39E(ATCC 33223)(一种嗜热,还原Fe(Ⅲ)的细菌)进行了评估,它具有从四个电子供体(木糖,葡萄糖,纤维二糖和乙酸盐)产生的电流的能力,且阳极电位固定( + 0.042 V vs.SHE)在微生物电化学电池(MXC)中。在嗜热条件下(60℃),假二乙醇可产生木糖(5.8±2.4 A m〜(-2)),葡萄糖(4.3±1.9 A m〜(-2))和纤维二糖(5.2±1.6)的高电流密度。 m〜(-2))。当与乙酸盐一起生长时,它产生的电流很小,但是消耗了糖发酵产生的乙酸盐以产生电流。低扫描循环伏安法(LSCV)显示S型响应,相对于SHE的中点电位为-0.17V。库仑效率(CE)因电子供体而异,木糖为34.8%±0.7%,葡萄糖为65.3%±1.0%,纤维二糖为27.7%±1.5%。阳极呼吸在5.4-8.3的pH范围内持续,在较高的pH值下观察到较高的电流密度。扫描电子显微镜在阳极上显示出一个完善的假单胞菌生物膜,而共聚焦激光扫描显微镜显示,葡萄糖喂养的生物膜的最大生物膜厚度(L_f)大于〜150μm。

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  • 来源
    《Environmental Science & Technology》 |2015年第24期|14725-14731|共7页
  • 作者单位

    Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, Arizona 85287-5701, United States;

    Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, Arizona 85287-5701, United States,Department of Microbiology, Quaid-Ⅰ-Azam University, Islamabad, 45320, Pakistan;

    Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, Arizona 85287-5701, United States,Department of Civil Engineering, Kansas State University, Manhattan, 2123 Fiedler Hall, Manhattan, Kansas 66502, United States;

    Department of Microbiology, Quaid-Ⅰ-Azam University, Islamabad, 45320, Pakistan;

    Department of Microbiology, Quaid-Ⅰ-Azam University, Islamabad, 45320, Pakistan;

    Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, Arizona 85287-5701, United States;

    Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, Arizona 85287-5701, United States,School for Engineering of Matter, Transport and Energy, Arizona State University, 501 E Tyler Mall, Tempe, Arizona 85287, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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