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首页> 外文期刊>Environmental Science & Technology >Genomic Characterization of Dehalococcoides mccartyi Strain JNA That Reductively Dechlorinates Tetrachloroethene and Polychlorinated Biphenyls
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Genomic Characterization of Dehalococcoides mccartyi Strain JNA That Reductively Dechlorinates Tetrachloroethene and Polychlorinated Biphenyls

机译：Dehalococcoides mccartyi菌株JNA的基因组表征，该菌株可还原性脱除四氯乙烯和多氯联苯。

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摘要

Dehalococcoides mccartyi strain JNA detoxifies highly chlorinated polychlorinated biphenyl (PCB) mixtures via 85 distinct dechlorination reactions, suggesting that it has great potential for PCB bioremediation. However, its genomic and functional gene information remain unknown due to extremely slow growth of strain JNA with PCBs. In this study, we used tetracholorethene (PCE) as an alternative electron acceptor to grow sufficient biomass of strain JNA for subsequent genome sequencing and functional gene identification. Analysis of the assembled draft genome (1462 509 bp) revealed the presence of 29 putative reductive dehalogenase (RDase) genes. Among them, JNA_RD8 and JNA_RD11 genes were highly transcribed in both PCE- and PCB-fed cultures. Furthermore, in vitro assays with crude cell lysate from PCE grown cells revealed dechlorination activity against both PCE and 2,2',3,4,4',5,5'-heptachlorobiphenyl. These data suggest that both JNA_RD8 and JNA_RD11 may be bifunctional PCE/PCB RDases. This study deepens the knowledge of organohalide respiration of PCBs and facilitates in situ PCB- bioremediation with strain JNA.
机译：Dehalococcoides mccartyi菌株JNA通过85个不同的脱氯反应对高度氯化的多氯联苯（PCB）混合物进行了解毒，这表明它具有很大的PCB生物修复潜力。但是，由于带有PCB的JNA菌株生长极其缓慢，其基因组和功能基因信息仍然未知。在这项研究中，我们使用胆甾烯（PCE）作为替代电子受体来生长菌株JNA的足够生物量，以用于随后的基因组测序和功能基因鉴定。分析组装的草图基因组（1462 509 bp），发现存在29个假定的还原性脱卤素酶（RDase）基因。其中，JNA_RD8和JNA_RD11基因在PCE和PCB喂养的培养物中均被高度转录。此外，用来自PCE生长的细胞的粗细胞裂解物进行的体外测定显示出对PCE和2,2'，3,4,4'，5,5'-七氯联苯的脱氯活性。这些数据表明JNA_RD8和JNA_RD11都可能是双功能PCE / PCB RDase。这项研究加深了PCBs的有机卤化物呼吸的知识，并促进了JNA菌株的原位PCB生物修复。

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  • 来源
    《Environmental Science & Technology》 |2015年第24期|14319-14325|共7页
  • 作者

    Shanquan Wang; Kern Rei Chng; Chen Chen; Donna L. Bedard; Jianzhong He;

  • 作者单位

    Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576,Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Ave., Urbana Illinois L61802, United States;

    Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576,Computational and Systems Biology, Genome Institute of Singapore, Singapore 138672;

    Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576;

    Department of Biological Sciences, Rensselaer Polytechnic Institute, 110 Eighth St., Troy, New York 12180, United States;

    Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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