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首页> 外文期刊>Environmental Science & Technology >Metabolomic Responses of Green Alga Chlamydomonas reinhardtii Exposed to Sublethal Concentrations of Inorganic and Methylmercury
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Metabolomic Responses of Green Alga Chlamydomonas reinhardtii Exposed to Sublethal Concentrations of Inorganic and Methylmercury

机译：绿藻衣原体Reinhardtii暴露于无机和甲基汞的亚甲酸浓度的代谢物反应

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摘要

Metabolomics characterizes low-molecular-weight molecules involved in different biochemical reactions and provides an integrated assessment of the physiological state of an organism. By using liquid chromatography-mass spectrometry targeted metabolomics, we examined the response of green alga Chlamydomonas reinhardtii to sublethal concentrations of inorganic mercury (IHg) and monomethylmercury (MeHg). We quantified the changes in the levels of 93 metabolites preselected based on the disturbed metabolic pathways obtained in a previous transcriptomics study. Metabolites are downstream products of the gene transcription; hence, metabolite quantification provided information about the biochemical status of the algal cells exposed to Hg compounds. The results showed that the alga adjusts its metabolism during 2 h exposure to 5 × 10~(-9) and 5 × 10~(-8) mol L~(-1) IHg and MeHg by increasing the level of various metabolites involved in amino acid and nucleotide metabolism, photorespiration, and tricarboxylic acid (TCA) cycle, as well as the metabolism of fatty acids, carbohydrates, and antioxidants. Most of the metabolic perturbations in the alga were common for IHg and MeHg treatments. However, the exposure to IHg resulted in more pronounced perturbations in the fatty acid and TCA metabolism as compared with the exposure to MeHg. The observed metabolic perturbations were generally consistent with our previously published transcriptomics results for C. reinhardtii exposed to the comparable level of IHg and MeHg. The results highlight the potential of metabolomics for toxicity evaluation, especially to detect effects at an early stage of exposure prior to their physiological appearance.
机译：代谢组学表征参与不同的生化反应的低分子量分子，并提供一个有机体的生理状态的综合评估。通过使用液相色谱 - 质谱法靶向代谢物组学，我们检查绿藻莱茵衣藻的无机汞（IHG）和monomethylmercury（甲基汞）的亚致死浓度的响应。我们量化在93种代谢物水平的变化预选的基础上在先前的转录组研究中获得的干扰代谢途径。代谢物是该基因转录的下游产品;因此，代谢物的定量提供了关于暴露于汞化合物藻细胞的生化状态信息。结果表明，所述藻类通过增加参与各种代谢物的水平期间2小时曝光调整其代谢到5×10〜（-9）和5×10〜（-8）摩尔〜（-1）IIIg的和甲基汞氨基酸和核苷酸代谢，光呼吸作用，和三羧酸（TCA）循环，以及脂肪酸，碳水化合物，和抗氧化剂的代谢。大多数藻类代谢紊乱的是洲际酒店集团和甲基汞治疗常见。然而，在暴露于IIIg的导致了脂肪酸和TCA代谢更明显的扰动，与暴露于甲基汞比较。与我们之前发表的转录组结果莱茵衣藻暴露洲际酒店和甲基汞的水平相当观察到的代谢紊乱普遍一致的。该结果强调代谢的毒性评价的潜力，特别是与现有其生理外观检测在曝光的早期阶段的影响。

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  • 来源
    《Environmental Science & Technology》 |2021年第6期|3876-3887|共12页
  • 作者

    Vera I. Slaveykova; Sanghamitra Majumdar; Nicole Regier; Weiwei Li; Arturo A. Keller;

  • 作者单位

    Faculty of Sciences Earth and Environment Sciences Department F.-A. Forel for Environmental and Aquatic Sciences Environmental Biogeochemistry and Ecotoxicology University of Geneva Geneva CH 1211 Switzerland;

    Bren School of Environmental Science & Management University of California Santa Barbara Santa Barbara California 93106-5131 United States;

    Faculty of Sciences Earth and Environment Sciences Department F.-A. Forel for Environmental and Aquatic Sciences Environmental Biogeochemistry and Ecotoxicology University of Geneva Geneva CH 1211 Switzerland;

    Bren School of Environmental Science & Management University of California Santa Barbara Santa Barbara California 93106-5131 United States;

    Bren School of Environmental Science & Management University of California Santa Barbara Santa Barbara California 93106-5131 United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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