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首页> 外文期刊>Environmental Science & Technology >Identification of Photosynthesis Inhibitors of Pelagic Marine Algae Using 96-Well Plate Microfractionation for Enhanced Throughput in Effect-Directed Analysis
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Identification of Photosynthesis Inhibitors of Pelagic Marine Algae Using 96-Well Plate Microfractionation for Enhanced Throughput in Effect-Directed Analysis

机译：使用96孔板微分馏技术鉴定远洋藻类的光合作用抑制剂，以提高产量

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摘要

Because of large-scale production and use of an increasing diversity of chemicals in modern society, estuarine and coastal waters may be contaminated with numerous substances. Some of these compounds have the potential to affect microalgae at the base of the pelagic food chain. Therefore, we identified the main chemical stressors that negatively affect the effective photosystem Ⅱ efficiency (φPSⅡ) in marine microalgae of the Dutch estuarme and coastal waters. An enhanced effect-directed analysis (EDA) was carried out by combining revened-phase ultra performance liquid chromatography fractionation of extracts from passive samplers, followed by effect assessment using the pulse amplitude modulation fluorometry assay and chemical analysis of biologically active fractions using high-resolution mass spectrometry. This study focuses on a novel microfractionation technique using 96-well plates to enhance throughput in EDA, structure elucidation, and the analytical and effect confirmation of the compounds that are identified. Although there are numerous unknown compounds present in estuarine and coastal waters, our EDA study shows that atrazine, diuron, irgarol, isoproturon, terbntryn, and terbutylazine are the main contributors to the observed effect on the φPSⅡ of marine microalgae.
机译：由于现代社会中大规模生产和使用越来越多的化学药品，河口和沿海水域可能被许多物质污染。这些化合物中的某些可能会影响远洋食物链底部的微藻。因此，我们确定了对荷兰河口和沿海水域微藻类有效光系统Ⅱ效率（φPSⅡ）产生负面影响的主要化学应激源。通过结合被动采样器提取物的反相超高效液相色谱分级分离，随后使用脉冲幅度调制荧光分析法进行效果评估以及使用高分辨率的生物活性组分的化学分析，进行了增强的效果导向分析（EDA）质谱。这项研究集中在使用96孔板的新型微分离技术上，以提高EDA的通量，结构阐明以及所鉴定化合物的分析和效果确认。尽管河口和沿岸水域中存在许多未知化合物，但我们的EDA研究表明，阿特拉津，杜伦，irgarol，异丙隆，叔丁烯和叔丁嗪是观察到的对海洋微藻φPSⅡ影响的主要贡献者。

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  • 来源
    《Environmental Science & Technology》 |2014年第14期|8003-8011|共9页
  • 作者

    Petra Booij; A. Dick Vethaak; Pim E. G. Leonards; Sascha B. Sjollema; Jeroen Kool; Pim de Voogt; Marja H. Lamoree;

  • 作者单位

    Institute for Environmental Studies, VU University, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;

    Institute for Environmental Studies, VU University, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands,Deltares, Marine and Coastal Systems, P.O. Box 177, 2600 MH Delft, The Netherlands;

    Institute for Environmental Studies, VU University, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;

    Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands;

    Faculty of Sciences, VU University, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;

    Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands,KWR Watercycle Research Institute, P.O. Box 1072, 3430 BB Nieuwegein, The Netherlands;

    Institute for Environmental Studies, VU University, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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