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首页> 外文期刊>Environmental Science & Technology >Multihapten Approach Leading to a Sensitive ELISA with Broad Cross-Reactivity to Microcystins and Nodularin
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Multihapten Approach Leading to a Sensitive ELISA with Broad Cross-Reactivity to Microcystins and Nodularin

机译：多半抗原方法导致对微囊藻毒素和结节霉素具有广泛交叉反应性的灵敏ELISA

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摘要

Microcystins (MCs) are a group of biotoxins (＞150) produced by cyanobacteria, with a worldwide distribution. MCs are hepatotoxic, and acute exposure causes severe liver damage in humans and animals. Rapid and cheap methods of analysis are therefore required to protect people and livestock, especially in developing countries. To include as many MCs as possible in a single analysis, we developed a new competitive ELISA. Ovine polyclonal antibodies were raised using an immunogen made by conjugating a mature of microcystins to cationised bovine serum albumin, and the plate-coating antigen was prepared by conjugating [Asp~3]MC- RY to ovalbumin. This strategy was used also to minimize specificity for particular microcystin congeners. Cross-reactivity studies indicate that the ELISA has broad specificity to microcystins and also detects nodularin, providing a sensitive and rapid analytical method for screening large numbers of samples. The limit of quantitation for microcystins in drinking water is 0.04 μg/L, well below the WHO's maximum recommendation of 1 μg/L. The ELISA can be used for quantifying total microcystins in various matrices, including drinking water, cyanobacterial cultures, extracts, and algal blooms, and may be useful in detecting metabolites and conjugates of MCs.
机译：微囊藻毒素（MCs）是蓝细菌产生的一组生物毒素（＞ 150），分布于世界各地。 MC具有肝毒性，急性暴露会对人和动物造成严重的肝损害。因此，需要快速而廉价的分析方法来保护人畜，尤其是在发展中国家。为了在一次分析中包括尽可能多的MC，我们开发了一种新的竞争性ELISA。使用通过将成熟的微囊藻毒素与阳离子化的牛血清白蛋白缀合而成的免疫原来产生绵羊多克隆抗体，并通过将[Asp〜3] MC-RY与卵白蛋白缀合来制备板包被抗原。该策略还用于最大程度降低对特定微囊藻毒素同源物的特异性。交叉反应研究表明，ELISA对微囊藻毒素具有广泛的特异性，还可以检测结节蛋白，从而为筛选大量样品提供了灵敏而快速的分析方法。饮用水中微囊藻毒素的定量极限为0.04μg/ L，远低于WHO的最大建议值1μg/ L。 ELISA可用于定量各种基质（包括饮用水，蓝细菌培养物，提取物和藻华）中的总微囊藻毒素，并可用于检测MC的代谢物和结合物。

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  • 来源
    《Environmental Science & Technology》 |2014年第14期|8035-8043|共9页
  • 作者

    Ingunn A. Samdal; Andreas Ballot; Kjersti E. Lovberg; Christopher O. Miles;

  • 作者单位

    Norwegian Veterinary Institute, P.O. Box 750, Sentrum, N-0106 Oslo, Norway;

    Norwegian Veterinary Institute, P.O. Box 750, Sentrum, N-0106 Oslo, Norway,Norwegian Institute for Water Research, Gaustadalleen 21, N-0349 Oslo, Norway;

    Norwegian Veterinary Institute, P.O. Box 750, Sentrum, N-0106 Oslo, Norway;

    Norwegian Veterinary Institute, P.O. Box 750, Sentrum, N-0106 Oslo, Norway,Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, N-0316 Oslo, Norway;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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