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首页> 外文期刊>Environmental Science & Technology >Molecular Analysis for Screening Human Bacterial Pathogens in Municipal Wastewater Treatment and Reuse
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Molecular Analysis for Screening Human Bacterial Pathogens in Municipal Wastewater Treatment and Reuse

机译：筛选城市污水处理和回用中人类细菌病原体的分子分析

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摘要

Eifective and sensitive monitoring of human pathogenic bacteria in municipal wastewater treatment is important not only for managing public health risk related to treated wastewater reuse, but also for ensuring proper functioning of the treatment plant. In this study, three different 16S rRNA gene molecular analysis methodologies were employed to screen bacterial pathogens in samples collected at three different stages of an activated sludge plant. Overall bacterial diversity was analyzed using next generation sequencing (NGS) on the Illumina MiSeq platform, as well as PCR-DGGE followed by band sequencing. In addition, a microdiversity analysis was conducted using PCR-DGGE, targeting Escherichia coli. Bioinformatics analysis was performed using QIIME protocol by clustering sequences against the Human Pathogenic Bacteria Database. NGS data were also clustered against the Greengenes database for a genera-level diversity analysis. NGS proved to be the most effective approach screening the sequences of 21 potential human bacterial pathogens, while the E coli microdiversity analysis yielded one (O157:H7 str. EDL933) out of the two E. coli strains picked up by NGS. Overall diversity using PCR-DGGE did not yield any pathogenic sequence matches even though a number of sequences matched the NGS results. Overall, sequences of Gram-negative pathogens decreased in relative abundance along the treatment train while those of Gram-positive pathogens increased.
机译：对市政污水处理中的人类致病细菌进行有效和敏感的监测，不仅对于管理与处理后的废水回用有关的公共健康风险非常重要，而且对于确保污水处理厂的正常运转也很重要。在这项研究中，采用三种不同的16S rRNA基因分子分析方法来筛选活性污泥厂三个不同阶段收集的样品中的细菌病原体。使用Illumina MiSeq平台上的下一代测序（NGS）以及PCR-DGGE，然后进行条带测序，分析了总体细菌多样性。另外，使用PCR-DGGE靶向大肠杆菌进行了微多样性分析。使用QIIME规程，通过将序列与人类病原细菌数据库进行聚类来进行生物信息学分析。 NGS数据也根据Greengenes数据库进行了聚类，以进行属级多样性分析。 NGS被证明是筛选21种潜在人类细菌病原体序列的最有效方法，而大肠杆菌微多样性分析从NGS挑选的两株大肠杆菌中产生了一种（O157：H7 str。EDL933）。使用PCR-DGGE进行的总体多样性分析未产生任何致病序列，即使许多序列与NGS结果匹配也是如此。总体而言，革兰氏阴性病原体的序列沿处理序列的相对丰度下降，而革兰氏阳性病原体的序列增加。

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  • 来源
    《Environmental Science & Technology》 |2014年第19期|11610-11619|共10页
  • 作者

    Rajkumari Kumaraswamy; Yamrot M. Amha; Muhammad Z. Anwar; Andreas Henschel; Jorge Rodriguez; Farrukh Ahmad;

  • 作者单位

    Institute Center for Water and Environment (iWATER), Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, UAE;

    Institute Center for Water and Environment (iWATER), Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, UAE;

    Institute Center for Smart and Sustainable Systems (iSmart), Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, UAE;

    Institute Center for Smart and Sustainable Systems (iSmart), Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, UAE;

    Institute Center for Water and Environment (iWATER), Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, UAE;

    Institute Center for Water and Environment (iWATER), Masdar Institute of Science and Technology, P.O. Box 54224, Abu Dhabi, UAE;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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