...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Toxicity of Drinking Water Disinfection Byproducts: Cell Cycle Alterations Induced by the Monohaloacetonitriles
【24h】

Toxicity of Drinking Water Disinfection Byproducts: Cell Cycle Alterations Induced by the Monohaloacetonitriles

机译：饮用水消毒副产物的毒性：单卤代乙腈诱导的细胞周期改变

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Haloacetonitriles (HANs) are a chemical class of drinking water disinfection byproducts (DBPs) that form from reactions between disinfectants and nitrogen-containing precursors, the latter more prevalent in water sources impacted by algae bloom and municipal wastewater effluent discharge. HANs, previously demonstrated to be genotoxic, were investigated for their effects on the mammalian cell cycle. Treating Chinese hamster ovary (CHO) cells with monoHANs followed by the release from the chemical treatment resulted in the accumulation of abnormally high DNA content in cells over time (hyperploid). The potency for the cell cycle alteration followed the order: iodoacetonitrile (IAN) ＞ bromoacetonitrile (BAN) ＞＞ chloroacetonitrile (CAN). Exposure to 6 μM IAN, 12 μM BAN and 900 μM CAN after 26 h post-treatment incubation resulted in DNA repair; however, subsequent cell cycle alteration effects were observed. Cell proliferation of HAN-treated cells was suppressed for as long as 43 to 52 h. Enlarged cell size was observed after 52 h post-treatment incubation without the induction of cytotoxicity. The HAN-mediated cell cycle alteration was mitosis- and proliferation-dependent, which suggests that HAN treatment induced mitosis override, and that HAN-treated cells proceeded into S phase and directly into the next cell cycle. Cells with multiples genomes would result in aneuploidy (state of abnormal chromosome number and DNA content) at the next mitosis since extra centrosomes could compromise the assembly of bipolar spindles. There is accumulating evidence of a transient tetraploid state proceeding to aneuploidy in cancer progression. Biological self-defense systems to ensure genomic stability and to eliminate tetraploid cells exist in eukaryotic cells. A key tumor suppressor gene, p53, is oftentimes mutated in various types of human cancer. It is possible that HAN disruption of the normal cell cycle and the generation of aberrant cells with an abnormal number of chromosomes may contribute to cancer induction and perhaps be involved in the induction of adverse pregnancy outcomes associated with long-term consumption of disinfected water. Here we present the first observation of the induction of hyperploidy by a class of DBPs.
机译：卤乙腈（HANs）是化学类别的饮用水消毒副产物（DBP），它是由消毒剂和含氮前体之间的反应形成的，后者在藻类大量繁殖和城市废水排放影响下的水源中更为普遍。以前被证明具有遗传毒性的HANs被研究对哺乳动物细胞周期的影响。用monoHANs处理中国仓鼠卵巢（CHO）细胞，然后从化学处理中释放出来，导致一段时间内细胞中异常高的DNA含量积聚（超倍体）。细胞周期改变的效力依序为：碘乙腈（IAN）＞溴乙腈（BAN）＞＞氯乙腈（CAN）。处理后孵育26小时后，暴露于6μMIAN，12μMBAN和900μMCAN导致DNA修复；然而，随后观察到细胞周期改变的影响。 HAN处理细胞的细胞增殖被抑制长达43至52小时。处理后孵育52小时后观察到细胞增大，而未诱导细胞毒性。 HAN介导的细胞周期改变是有丝分裂和增殖依赖性的，这表明HAN处理诱导的有丝分裂被超越，并且HAN处理过的细胞进入S期并直接进入下一个细胞周期。具有多个基因组的细胞会在下一个有丝分裂时导致非整倍性（染色体数目和DNA含量异常的状态），因为额外的中心体可能会损害双极纺锤体的组装。有越来越多的证据表明短暂的四倍体状态会在癌症进展中发展为非整倍性。确保基因组稳定性和消除四倍体细胞的生物自卫系统存在于真核细胞中。关键的肿瘤抑制基因p53通常在各种类型的人类癌症中发生突变。 HAN可能会破坏正常细胞周期的HAN和染色体数目异常的异常细胞的产生，可能有助于癌症的诱发，并且可能与长期饮用消毒水有关的不良妊娠结局的诱发。在这里，我们介绍由一类DBP诱导超倍性的首次观察。

著录项

  • 来源
    《Environmental Science & Technology》 |2014年第19期|11662-11669|共8页
  • 作者

    Yukako Komaki; Benito J. Marinas; Michael J. Plewa;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States, Center of Advanced Materials for the Purification of Water with Systems, Safe Global Water Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States, Center of Advanced Materials for the Purification of Water with Systems, Safe Global Water Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States;

    Center of Advanced Materials for the Purification of Water with Systems, Safe Global Water Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States, Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号