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首页> 外文期刊>Environmental Science & Technology >Bacterial Attachment and Viscoelasticity: Physicochemical and Motility Effects Analyzed Using Quartz Crystal Microbalance with Dissipation (QCM-D)
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Bacterial Attachment and Viscoelasticity: Physicochemical and Motility Effects Analyzed Using Quartz Crystal Microbalance with Dissipation (QCM-D)

机译:细菌附着和粘弹性:利用耗散的石英晶体微天平(QCM-D)分析的理化和动力效应

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摘要

This investigation is focused on the combined effect of bacterial physicochemical characteristics and motility on cell adhesion and deposition using a flow-through quartz crystal microbalance with dissipation (QCM-D). Three model flagellated strains with different degrees of motility were selected, including a highly motile Escherichia coli K12 MG1655, an environmental strain Sphingomonas wittichii RW1, and a nonmotile (with paralyzed flagella) Escherichia coli K12 MG1655 ΔmotA that is incapable of encoding the motor torque generator for flagellar movement. Of the three strains, S. wittichii RW1 is highly hydrophobic, while E. coli strains are equally hydrophilic. Consideration of the hydrophobicity provides an alternative explanation for the bacterial adhesion behavior. QCM-D results show that motility is a critical factor in determining bacterial adhesion, as long as the aquatic chemical conditions are conducive for motility and the substratum and bacterial surface are similarly hydrophobic or hydrophilic. Once their properties are not similar, the contribution of hydrophobic interactions becomes more pronounced. QCM-D results suggest that during adhesion of the hydrophobic bacterium, S. wittichii RW1, the initial step of adhesion and maturation of bacteria-substratum interaction on hydrophilic surface includes a dynamic change of the viscoelastic properties of the bond bacterium-surface becoming more viscously oriented.
机译:这项研究的重点是使用带耗散的流通式石英晶体微天平,结合细菌理化特性和运动性对细胞粘附和沉积的综合影响。选择了三种具有不同运动度的带鞭毛模型菌株,包括高能动性大肠杆菌K12 MG1655,环境菌株Sphingomonas wittichii RW1和非能动性(鞭毛瘫痪)大肠杆菌K12 MG1655ΔmotA,它们无法编码电动机转矩产生器鞭毛运动。在这三种菌株中,维氏链球菌RW1具有高度疏水性,而大肠杆菌菌株具有同样的亲水性。考虑疏水性为细菌粘附行为提供了另一种解释。 QCM-D结果表明,只要水生化学条件有利于运动,并且基质和细菌表面具有相似的疏水性或亲水性,运动性便是决定细菌粘附的关键因素。一旦它们的性质不相似,疏水相互作用的作用就变得更加明显。 QCM-D结果表明,在疏水细菌S. wittichii RW1粘附过程中,细菌与基质之间在亲水表面上的粘附和成熟的初始步骤包括键细菌表面的粘弹性的动态变化变得更加粘稠。面向。

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  • 来源
    《Environmental Science & Technology》 |2013年第1期|398-404|共7页
  • 作者单位

    Department of Desalination and Water Treatment, Zuckerberg Institute for Water Research, Albert Katz International School for Desert Studies, Jacob Blaustein Institute for Desert Research, Ben Gurion University of the Negev, Israel 84990;

    Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, United States;

    The Wolfson Department of Chemical Engineering, Technion - Israel Institute of Technology, Haifa 32000, Israel;

    Department of Desalination and Water Treatment, Zuckerberg Institute for Water Research, Albert Katz International School for Desert Studies, Jacob Blaustein Institute for Desert Research, Ben Gurion University of the Negev, Israel 84990;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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