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首页> 外文期刊>Environmental Science & Technology >Triclosan Impairs Swimming Behavior and Alters Expression of Excitation-Contraction Coupling Proteins in Fathead Minnow (Pimephales promelas)
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Triclosan Impairs Swimming Behavior and Alters Expression of Excitation-Contraction Coupling Proteins in Fathead Minnow (Pimephales promelas)

机译：三氯生损害游泳行为并改变黑头Fat（Pimephales promelas）中的兴奋-收缩偶联蛋白的表达

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摘要

Triclosan (TCS), a high volume chemical widely used in consumer products, is a known aquatic contaminant found in fish inhabiting polluted watersheds. Mammalian studies have recently demonstrated that TCS disrupts signaling between the ryanodine receptor (RyR) and the dihydropyridine receptor (DHPR), two proteins essential for excitation-contraction (EC) coupling in striated muscle. We investigated the swimming behavior and expression of EC coupling proteins in larval fathead minnows (Pimephales promelas) exposed to TCS for up to 7 days. Concentrations as low as 75 μg L~(-1) significantly altered fish swimming activity after 1 day; which was consistent after 7 days of exposure. The mRNA transcription and protein levels of RyR and DHPR (subunit CaVl.l) isoforms changed in a dose and time dependent manner. Crude muscle homogenates from exposed larvae did not display any apparent changes in receptor affinity toward known radioligands. In nonexposed crude muscle homogenates, TCS decreased the binding of [~3H]PN20-110 to the DHPR and decreased the binding of [~3H]- ryanodine to the RyR, demonstrating a direct impact at the receptor level. These results support TCS's impact on muscle function in vertebrates further exemplifying the need to re-evaluate the risks this pollutant poses to aquatic environments.
机译：三氯生（TCS）是消费品中广泛使用的一种高容量化学物质，是在居住于受污染流域的鱼类中发现的一种已知水生污染物。哺乳动物的研究最近表明，TCS会破坏条纹肌中兴奋收缩（EC）耦合所必需的两种蛋白质-ryanodine受体（RyR）和二氢吡啶受体（DHPR）之间的信号传导。我们调查了暴露于TCS长达7天的幼鱼黑头小鱼（Pimephales promelas）的游泳行为和EC偶联蛋白的表达。 1天后，低至75μgL〜（-1）的浓度会显着改变鱼的游泳活动；接触7天后保持一致。 RyR和DHPR（亚基CaV1.1）亚型的mRNA转录和蛋白质水平以剂量和时间依赖性方式改变。来自暴露幼虫的粗肌肉匀浆在对已知放射性配体的受体亲和力上未显示任何明显变化。在未暴露的粗肌肉匀浆中，TCS降低了[〜3H] PN20-110与DHPR的结合，并降低了[〜3H]-ryanodine与RyR的结合，表明了对受体水平的直接影响。这些结果支持了TCS对脊椎动物肌肉功能的影响，进一步说明了需要重新评估该污染物对水生环境构成的风险。

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  • 来源
    《Environmental Science & Technology》 |2013年第4期|2008-2017|共10页
  • 作者

    Erika B. Fritsch; Richard E. Connon; Inge Werner; Rebecca E. Davies; Sebastian Beggel; Wei Feng; Isaac N. Pessah;

  • 作者单位

    Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California 95616, United States;

    Deptartment of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, California 95616, United States;

    Swiss Centre for Applied Ecotoxicolgy, Ewag-EPFL, Dubendorf, Switzerland;

    Deptartment of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, California 95616, United States;

    Aquatic Systems Biology, Department of Ecology, Technische Universitat Miinchen, Freising, Germany;

    Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California 95616, United States;

    Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California 95616, United States,The Medical Investigations of Neurodevelopmental Disorders Institute, University of California Davis Medical Center, Sacramento, California, 95817, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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