...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Air-Drying Beds Reduce the Quantities of Antibiotic Resistance Genes and Class 1 Integrons in Residual Municipal Wastewater Solids
【24h】

Air-Drying Beds Reduce the Quantities of Antibiotic Resistance Genes and Class 1 Integrons in Residual Municipal Wastewater Solids

机译：空气干燥床减少了残留市政污水固体中抗生素抗性基因和1类整合素的数量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study investigated whether air-drying beds reduce antibiotic resistance gene (ARG) concentrations in residual municipal wastewater solids. Three laboratory-scale drying beds were operated for a period of nearly 100 days. Real-time PCR was used to quantify 16S rRNA genes, 16S rRNA genes specific to fecal bacteria (AllBac) and human fecal bacteria (HF183), the integrase gene of class 1 integrons (intI1), and five ARGs representing a cross-section of antibiotic classes and resistance mechanisms (erm(B), sulI, tet(A), tet(W), and tet(X)). Air-drying beds were capable of reducing all gene target concentrations by 1 to 5 orders of magnitude, and the nature of this reduction was consistent with both a net decrease in the number of bacterial cells and a lack of selection within the microbial community. Half-lives varied between 1.5 d (HF183) and 5.4 d (tet(X)) during the first 20 d of treatment After the first 20 d of treatment, however, half-lives varied between 8.6 d (tet(X)) and 19.3 d (AllBac), and 16S rRNA gene, intI1, and sul1 concentrations did not change (P ＞ 0.05). These results demonstrate that air-drying beds can reduce ARG and intI1 concentrations in residual municipal wastewater solids within timeframes typical of operating practices.
机译：这项研究调查了风干床是否降低了残留市政污水中的抗生素抗性基因（ARG）浓度。三个实验室规模的干燥床运行了将近100天。实时荧光定量PCR用于定量16S rRNA基因，粪便细菌（AllBac）和人粪便细菌（HF183）特有的16S rRNA基因，1类整合素的整合酶基因（intI1）和代表ASC横截面的5个ARG。抗生素类别和耐药机制（erm（B），sulI，tet（A），tet（W）和tet（X））。空气干燥床能够将所有基因靶标浓度降低1-5个数量级，并且这种降低的性质与细菌细胞数量的净减少和微生物群落内缺乏选择相一致。在治疗的前20天内，半衰期在1.5 d（HF183）和5.4 d（tet（X））之间变化，但是在治疗的前20 d后，半衰期在8.6 d（tet（X））和6.5 d（tet（X））之间变化。 19.3 d（AllBac）和16S rRNA基因，intI1和sul1浓度未改变（P＞ 0.05）。这些结果表明，风干床可以在典型的操作时间范围内降低残留市政废水中的ARG和intI1浓度。

著录项

  • 来源
    《Environmental Science & Technology》 |2013年第17期|9965-9971|共7页
  • 作者

    Tucker R. Burch; Michael J. Sadowsky; Timothy M. LaPara;

  • 作者单位

    Department of Civil Engineering, University of Minnesota, Minneapolis, Minnesota 55455, United States;

    BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, United States,Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota 55108, United States;

    Department of Civil Engineering, University of Minnesota, Minneapolis, Minnesota 55455, United States,BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号