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首页> 外文期刊>Environmental Science & Technology >Fate of Antibiotic Resistance Genes and Class 1 Integrons in Soil Microcosms Following the Application of Treated Residual Municipal Wastewater Solids
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Fate of Antibiotic Resistance Genes and Class 1 Integrons in Soil Microcosms Following the Application of Treated Residual Municipal Wastewater Solids

机译:应用处理后的残留城市污水固体后土壤微观世界中抗生素抗性基因和1类整合子的命运

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摘要

Substantial quantities of antibiotic resistance genes (ARGs) are discharged with treated residual municipal wastewater solids and subsequently applied to soil. The objective of this work was to determine the decay rates for ARGs and class 1 integrons following simulated land application of treated wastewater solids. Treated residual solids from two full-scale treatment plants were applied to sets of triplicate soil microcosms in two independent experiments. Experiment 1 investigated loading rates of 20,40, and 100 g kg~(-1) of residual solids to a sandy soil, while experiment 2 investigated a loading rate of 40 g kg~(-1) to a silty-loamy soil Five ARGs (erm(B), sul1, tet(A), tet(W), and tet(X)), the integrase of class 1 integrons (intI1), 16S rRNA genes, 16S rRNA genes of all Bactcroides spp., and 16S rRNA genes of human-specific Bacteroides spp. were quantified using realtime polymerase chain reaction. ARGs and intI1 quantities declined in most microcosms, with statistically significant (P <0.05) half-lives varying between 13 d (erm(B), experiment 1,100 g kg~(-1)) and 81 d (intI1, experiment 1, 40 g kg~(-1)). These kinetic rates were much slower than have been previously reported for unit operations used to treat wastewater solids (e.g., anaerobic digestion). This research suggests that the design and operation of municipal wastewater treatment faculties with the explicit goal of mitigating the release of ARGs should focus on using technologies within the treatment facility, rather than depending on attenuation subsequent to land application.
机译:大量的抗生素抗性基因(ARG)与处理过的残留城市污水一起排放,然后施用于土壤。这项工作的目的是确定经过处理的废水固体在土地上的模拟之后ARG和1类整形的衰减率。在两个独立的实验中,将来自两个大型处理厂的处理过的残留固体应用于三组土壤微观世界。实验1研究了20,40和100 g kg〜(-1)的残余固体在沙质土壤中的负载率,而实验2研究了40 g kg〜(-1)的粉质壤土土壤的负载率5 ARGs(erm(B),sul1,tet(A),tet(W)和tet(X)),1类整合素(intI1),16S rRNA基因,所有Bactcroides spp。的16S rRNA基因的整合以及人类特异性拟杆菌的16S rRNA基因。使用实时聚合酶链反应定量。大多数微观世界中的ARGs和intI1数量均下降,半衰期在13 d(erm(B),实验1100 g kg〜(-1))和81 d(intI1,实验1,40)之间具有统计学意义(P <0.05)。克公斤〜(-1))。这些动力学速率比以前报道的用于处理废水固体(例如厌氧消化)的单元操作要慢得多。这项研究表明,以减轻ARGs释放为明确目标的市政废水处理学院的设计和运营应着重于在处理设施内使用技术,而不是依赖土地施用后的衰减。

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  • 来源
    《Environmental Science & Technology》 |2014年第10期|5620-5627|共8页
  • 作者单位

    Department of Civil Engineering, University of Minnesota, Minneapolis, Minnesota 55455, United States,Laboratory for Infectious Disease and the Environment, Wisconsin Water Science Center, U.S. Geological Survey, Marshfield, WI 54449, United States;

    BioTechnology Institute University of Minnesota, St. Paul, Minnesota 55108, United States,Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota 55108, United States;

    Department of Civil Engineering, University of Minnesota, Minneapolis, Minnesota 55455, United States,BioTechnology Institute University of Minnesota, St. Paul, Minnesota 55108, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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