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首页> 外文期刊>Environmental Science & Technology >Native Bacterial Community Convergence in Augmented and Stimulated Ureolytic MICP Biocementation
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Native Bacterial Community Convergence in Augmented and Stimulated Ureolytic MICP Biocementation

机译：天然细菌群落收敛在增强和刺激的Quolemytic MICP生物体

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摘要

Microbially induced calcite precipitation is a biomineraliza- tion process with numerous civil engineering and ground improvement applications. In replicate soil columns, the efficacy and microbial composition of soil bioaugmented with the ureolytic bacterium Sporosarcina pasteurii were compared to a biostimulation method that enriches native ureolytic soil bacteria in situ under conditions analogous to field implementation. The selective enrichment resulting from sequential stimulation treatments strongly selected for Firmicutes (＞97%), with Sporosarcina and Lysinibacillus comprising 60 to 94% of high-throughput 16S rDNA sequences in each suspended community sample. Seven species of the former and two of the latter were present in greater than 10% abundance at different times, demonstrating unexpected within-genus diversity and robustness in the suspended phase of this highly selective environment. Based on longer 16S sequences, it was inferred that augmented S. pasteurii competed poorly with natural bacteria, decreasing to below detection after nine treatments, while the native microbial community was enriched to approximately that present in the stimulated columns. These analyses were corroborated by the observed convergence in bulk ureolytic rates and calcite contents between techniques. However, a 10-fold discrepancy between the observed cell density and an activity-based estimate indicates the attached community, uncharacterized despite efforts, substantially contributes to bulk behavior.
机译：微生物诱导的方解石沉淀是一种具有许多土木工程和地面改进应用的生物纳米蛋白化过程。在重复的土壤柱中，将与ureolyticBacterium sporosarcina pasteurii的土壤生物化的疗效和微生物组成与生物刺激方法进行比较，其在类似于现场实施的条件下原位丰富天然尿液土壤细菌。由序贯刺激处理产生的选择性富集，强烈地选择用于燃料（> 97％），SporoSarcina和溶酶包含60-94％的高通量16s RDNA序列在每个悬浮的群落样本中。前者和后者的七种物种在不同时间大于10％的丰度，展示了这种高度选择性环境的悬浮阶段的意外内部多样性和鲁棒性。基于较长的16S序列，推断出于天然细菌的增强S. Pasteurii，在九种处理后降低低于检测，而原生微生物群落富集到刺激的柱中的含量约为。这些分析通过观察到的批量溶质率和方解石含量在技术之间进行了证实。然而，观察到的细胞密度和基于活性的估计之间的10倍差异表明了附加的社区，尽管努力尽管努力，但基本上有助于批量行为。

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  • 来源
    《Environmental Science & Technology》 |2021年第15期|10784-10793|共10页
  • 作者

    Charles M. R. Graddy; Michael G. Gomez; Jason T. DeJong; Douglas C. Nelson;

  • 作者单位

    Department of Microbiology and Molecular Genetics University of California Davis 95616 California United States;

    Department of Civil and Environmental Engineering University of Washington Seattle 98195-2700 Washington United States;

    Department of Civil and Environmental Engineering University of California Davis 95616 California United States;

    Department of Microbiology and Molecular Genetics University of California Davis 95616 California United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biogeotechnics; biogrout; bio-mediated; bio-stimulation; bio-augmentation; calcite; carbonate; Sporosarcina pasteurii;

    机译：生物质地耳塞;生物收录;生物介导;生物刺激;生物增强;方解石;碳酸盐;SporoSarcina Pasteurii。;

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