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首页> 外文期刊>Environmental Science & Technology >Genome-Wide Bacterial Toxicity Screening Uncovers the Mechanisms of Toxicity of a Cationic Polystyrene Nanomaterial
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Genome-Wide Bacterial Toxicity Screening Uncovers the Mechanisms of Toxicity of a Cationic Polystyrene Nanomaterial

机译：全基因组细菌毒性筛选揭示了阳离子聚苯乙烯纳米材料的毒性机理。

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摘要

By exploiting a genome-wide collection of bacterial single-gene deletion mutants, we have studied the toxicological pathways of a 60-nm cationic (amino-function- alized) polystyrene nanomaterial (PS-NH_2) in bacterial cells. The IC_(50) of commercially available 60 run PS-NH_2 was determined to be 158 μg/mL, the IC_5 is 108 μg/mL, and the IC_(90) is 190 μg/mL for the parent E. coli strain of the gene deletion library. Over 4000 single nortessential gene deletion mutants of Escherichia coli were screened for the growth phenotype of each strain in the presence and absence of PS- NH_2. This revealed that genes clusters in the lipopolysacchar- ide biosynthetic pathway, outer membrane transport channels, ubiquinone biosynthetic pathways, flagellar movement, and DNA repair systems are all important to how this organism responds to cationic nanomaterials. These results, coupled with those from confirmatory assays described herein, suggest that the primary mechanisms of toxicity of the 60-nm PS-NH_2 nanomaterial in E. coli are destabilization of the outer membrane and production of reactive oxygen species. The methodology reported herein should prove generally useful for identifying pathways that are involved in how cells respond to a broad range of nanomaterials and for determining the mechanisms of cellular toxicity of different types of nanomaterials.
机译：通过利用全基因组范围内的细菌单基因缺失突变体的收集，我们研究了细菌细胞中60 nm阳离子（氨基官能化）聚苯乙烯纳米材料（PS-NH_2）的毒理学途径。经测定，市售60种PS-NH_2的IC_（50）为158μg/ mL，IC_5为108μg/ mL，而该菌株的亲本大肠杆菌菌株的IC_（90）为190μg/ mL。基因删除文库。在存在和不存在PS-NH_2的情况下，针对每种菌株的生长表型筛选了超过4000个大肠杆菌的单等义基因缺失突变体。这表明，脂多糖生物合成途径，外膜转运通道，泛醌生物合成途径，鞭毛运动和DNA修复系统中的基因簇对这种生物如何响应阳离子纳米材料至关重要。这些结果，加上本文所述的验证性试验的结果，表明60nm PS-NH_2纳米材料在大肠杆菌中的毒性的主要机理是外膜不稳定和活性氧的产生。本文报道的方法论应被证明通常可用于鉴定细胞如何响应广泛的纳米材料以及确定不同类型的纳米材料的细胞毒性机制的途径。

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  • 来源
    《Environmental Science & Technology》 |2012年第4期|p.2398-2405|共8页
  • 作者

    Angela Ivask; Elizabeth Suarez; Trina Patel; David Boren; Zhaoxia Ji; Patricia Holden; Donatello Telesca; Robert Damoiseaux; Kenneth A. Bradley; Hilary Godwin;

  • 作者单位

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,California NanoSystems Institute, University of California, Los Angeles, California 90095, United States,Laboratory of Molecular Genetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,California NanoSystems Institute, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,Department of Biostatistics, School of Public Health, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,Department of Biostatistics, School of Public Health, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,California NanoSystems Institute, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,Donald Bren School of Environmental Science and Management, University of California, Santa Barbara, California 93106, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,Department of Biostatistics, School of Public Health, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,California NanoSystems Institute, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,California NanoSystems Institute, University of California, Los Angeles, California 90095, United States,Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095, United States;

    University of California Center for Environmental Implications of Nanotechnology, University of California, Los Angeles, California 90095, United States,Institute of the Environment and Sustainability, University of California, Los Angeles, California 90095, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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