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首页> 外文期刊>Environmental Science & Technology >Novel Fiber Optic Detection Method for in Situ Analysis of Fluorescently Labeled Biosensor Organisms
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Novel Fiber Optic Detection Method for in Situ Analysis of Fluorescently Labeled Biosensor Organisms

机译：用于荧光标记生物传感器生物原位分析的新型光纤检测方法

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摘要

This research presents a novel, time-resolved fiber-optic "Optrode" system for accurate real-time in situ detection of fluorescent proteins produced by biosensor organisms. The Optrode fluorescence detection system was able to identify, characterize, differentiate, and quantify red and green fluorescently labeled organisms, individually and in mixed aqueous cultures. Detection was also possible in sand systems, where a consistent reduction in signal intensity indicates that signal collection volume was reduced by one-third. The optrode was shown to be sensitive enough to detect fluorescently labeled cell concentrations of 1.9 × 10~4 CFU/ mL, indicating it is suitable for detecting typical concentrations of degrader organisms reported in bioremediation trials. The effect of fluorophore photobleaching was characterized for different fluorescent proteins and demonstrated a linear relationship to cell concentration, meaning the effect can be accounted for within methods of fluorescence collection and analysis. Proof of concept is provided for all aspects of this research, which represents an important step toward the goal of achieving a complete, nondestructive, in situ monitoring system to characterize all aspects of microbial activity and gene expression.
机译：这项研究提出了一种新颖的，时间分辨的光纤“ Optrode”系统，用于精确实时地原位检测由生物传感器生物产生的荧光蛋白。 Optrode荧光检测系统能够分别，在混合水培中鉴定，表征，区分和定量红色和绿色荧光标记的生物。在沙子系统中也可以进行检测，其中信号强度持续降低表明信号收集量减少了三分之一。该电极具有足够的灵敏度，可以检测到1.9×10〜4 CFU / mL的荧光标记细胞浓度，这表明它适用于检测生物修复试验中报告的典型降解生物的浓度。荧光团光漂白的作用已针对不同的荧光蛋白进行了表征，并证明了其与细胞浓度呈线性关系，这意味着该作用可在荧光收集和分析方法中进行解释。为这项研究的所有方面提供了概念验证，这是朝着实现完整，无损，原位监测系统以表征微生物活性和基因表达各个方面的目标迈出的重要一步。

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  • 来源
    《Environmental Science & Technology》 |2012年第10期|p.5414-5421|共8页
  • 作者

    Belinda M. Hewitt; Naresh Singhal; Roy G. Elliot; Andy Y. H. Chen; Jason Y. C. Kuo; Frederique Vanholsbeeck; Simon Swift;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Auckland, New Zealand;

    Department of Civil and Environmental Engineering, University of Auckland, New Zealand;

    Department of Civil and Environmental Engineering, University of Auckland, New Zealand;

    Department of Physics, University of Auckland, New Zealand;

    Department of Physics, University of Auckland, New Zealand;

    Department of Physics, University of Auckland, New Zealand;

    Department of Molecular Medicine and Pathology, University of Auckland, New Zealand;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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