...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Structural and Functional Effects of Cu Metalloprotein-Driven Silver Nanoparticle Dissolution
【24h】

Structural and Functional Effects of Cu Metalloprotein-Driven Silver Nanoparticle Dissolution

机译：铜金属蛋白驱动的银纳米颗粒溶解的结构和功能效应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Interactions of a model Cu-metalloprotein,azurin,withrn10-100 run silver nanoparticles (NPs) were examined to elucidate thernrole of oxidative dissolution and protein interaction on the biologicalrnreactivity of NPs.Although minimal protein and NP structural changesrnwere observed upon interaction,displacement of Cu(II) and formation ofrnAg(I) azurin species under aerobic conditions implicates Cu(II) azurin asrna catalyst of NP oxidative dissolution.Consistent with NP oxidationrnpotentials,largest concentrations of Ag(I) azurin species were recorded inrnreaction with 10 nm NPs (>50%).Apo-protein was also observed underrnanaerobic reaction with NPs of all sizes and upon aerobic reaction with larger NPs (>20 nm),where NP oxidation is slowed.rnCu(II) azurin displacement upon reaction with NPs was significantly greater than when reacted with Ag(I)(aq) alone.Regardlessrnof NP size,dialysis experiments show minimal reactivity between azurin and the Ag(I)(aq) species formed as a result of NPrnoxidative dissolution,indicating Cu displacement from azurin occurs at the NP surface.Mechanisms of azurin-silver NPrninteraction are proposed.Results demonstrate that NP interactions not only impact protein structure and function,but also NPrnreactivity,with implications for targeting,uptake,and cytotcuricity.
机译：研究了模型铜金属蛋白，天青素与10-100纳米银纳米颗粒（NPs）的相互作用，以阐明氧化溶解和蛋白质相互作用对NPs生物反应性的作用。尽管在相互作用，铜的置换后观察到最小的蛋白质和NP结构变化（II）和好氧条件下rnAg（I）天青蛋白物种的形成暗示了NP（NP）氧化溶解的Cu（II）天青星asrna催化剂。与NP氧化潜能一致，记录到最大浓度的Ag（I）天青蛋白物种与10 nm NPs不反应（> 50％）。在与各种大小的NPs进行厌氧反应时，以及与较大NPs（> 20 nm）的需氧反应时，也观察到载脂蛋白，其中NP氧化减慢了。与NPs反应时rnCu（II）天青蛋白的位移明显大于与单独的Ag（I）（aq）反应时。不管NP大小如何，透析实验均显示天青素与由NPr形成的Ag（I）（aq）物种之间的反应性最小提出了金银蛋白-银NPrn相互作用的机理。结果表明，NP相互作用不仅影响蛋白质的结构和功能，而且还影响NP的反应性，对靶向，摄取和细胞毒性具有影响。

著录项

  • 来源
    《Environmental Science & Technology》 |2012年第11期|p.6355-6362|共8页
  • 作者

    Andrew J.Martinolich; Grace Park; Meagan Y.Nakamoto; Rachel E.Gate; Korin E.Wheeler;

  • 作者单位

    Department of Chemistry & Biochemistry,Santa Clara University,Santa Clara,California 95053 United States;

    Department of Chemistry & Biochemistry,Santa Clara University,Santa Clara,California 95053 United States;

    Department of Chemistry & Biochemistry,Santa Clara University,Santa Clara,California 95053 United States;

    Department of Chemistry & Biochemistry,Santa Clara University,Santa Clara,California 95053 United States;

    Department of Chemistry & Biochemistry,Santa Clara University,Santa Clara,California 95053 United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号