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Enrichment and Analysis of Anode-Respiring Bacteria from Diverse Anaerobic Inocula

机译:多种厌氧接种物中富集阳极呼吸细菌的分析

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摘要

One of the limitations currently faced by microbial electrochemical cell (MXC) technologies lies in the shortage of different organisms capable of forming a bionlm and channeling electrons from substrates to the anode at high current densities. Using a poised anode (-0.30 V vs Ag/AgCl) and acetate as the electron donor in a MXC, we demonstrated the presence of highly efficient anode-respiring bacteria (ARB) able to produce high current densities (>1.5 A/m~2 anode) in seven out of thirteen environmental samples. These included marshes, lake sediments, saline microbial mats, and anaerobic soils obtained from geographically diverse locations. Our microbial ecology analysis, using pyrosequenc-ing, shows that bacteria related to the genus Geobacter, a known and commonly found ARB, dominate only two of the bionlm communities producing high current; other bionlm communities contained different known and/or novel ARB. The presence of ARB in geographically diverse locations indicates that ARB thrive in a wide range of ecosystems. Studying ARB from different environmental conditions will allow us to better understand the ubiquity of anode respiration, compare the capabilities of different ARB consortia, and find ARB with useful metabolic capacities for future applications.
机译:微生物电化学电池(MXC)技术目前面临的局限性之一是缺乏能够形成仿生膜并在高电流密度下将电子从基板传输到阳极的不同生物。在MXC中使用平衡的阳极(-0.30 V vs Ag / AgCl)和醋酸盐作为电子供体,我们证明了能够产生高电流密度(> 1.5 A / m〜)的高效阳极呼吸细菌(ARB)的存在13个环境样品中的7个为阳极)。这些包括沼泽,湖泊沉积物,盐碱微生物垫和从不同地理位置获得的厌氧土壤。我们使用焦磷酸酶的微生物生态学分析表明,与地球细菌属有关的细菌(一种已知且常见的ARB)仅在两个产生高电流的生物群落中占主导地位。其他生物社区包含不同的已知和/或新颖的ARB。 ARB在不同地理位置的存在表明ARB在广泛的生态系统中蓬勃发展。从不同的环境条件研究ARB将使我们能够更好地了解阳极呼吸的普遍性,比较不同ARB财团的能力,并找到具有有用代谢能力的ARB以备将来使用。

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  • 来源
    《Environmental Science & Technology》 |2012年第18期|p.10349-10355|共7页
  • 作者单位

    Swette Center for Environmental Biotechnology, Biodesign Institute, Arizona State University, Tempe, Arizona, United States;

    Swette Center for Environmental Biotechnology, Biodesign Institute, Arizona State University, Tempe, Arizona, United States;

    Swette Center for Environmental Biotechnology, Biodesign Institute, Arizona State University, Tempe, Arizona, United States;

    School of Sustainable Engineering and the Built Environment, Arizona State University, 1001 South McAllister Avenue (location and courier service), P.O. Box 875701 (U.S. Postal Service only), Tempe, AZ 85287-5701 USA;

    School for Engineering of Matter, Transport and Energy, Swette Center for Environmental Biotechnology at Biodesign Institute, Arizona State University, 1001 South McAllister Avenue (location and courier service), P.O. Box 875701 (U.S. Postal Service only), Tempe, AZ 85287-S70I USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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