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首页> 外文期刊>Environmental Science & Technology >Mechanisms of Humic Acids Degradation by White Rot Fungi Explored Using 1H NMR Spectroscopy and FTICR Mass Spectrometry
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Mechanisms of Humic Acids Degradation by White Rot Fungi Explored Using 1H NMR Spectroscopy and FTICR Mass Spectrometry

机译：1H NMR光谱和FTICR质谱研究白腐真菌降解腐殖酸的机理

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摘要

Enzymatic activities involved in decay processes of natural aromatic macromolecules, such as humic acids (HA) and lignin by white rot fungi, have been widely investigated. However, the physical and chemical analysis of degradation products of these materials has not been intensively explored. Fourier transform cyclotron resonance mass spectrometry (FTICR MS) and *H NMR as well as CHNOS and size exclusion chromatography were employed to study the mechanisms of HA degradation by Trametes sp. M23 and Phaner-ochaete sp. Y6. Size exclusion chromatography analyses demonstrate and provide evidence for HA breakdown into low MW compounds. The *H NMR analysis revealed oxidation, a decrease in the aromatic content, and an indication of demethylation of the HA during biodegradation. Evidence for oxidation was also obtained using CHNOS. Analysis of FTICR MS results using a new software program developed by our group (David Mass Sort) revealed consecutive series of masses suggesting biochemical degradation trends such as oxidation, aromatic cleavage, and demethylation. These results are in agreement with the lH NMR analysis and with the suggested role of the hgninolytic system leading to HA degradation.
机译：已经广泛研究了涉及天然芳香族大分子诸如腐殖酸（HA）和木质素的腐烂过程中由白腐真菌引起的酶活性。然而，尚未深入研究这些材料的降解产物的物理和化学分析。用傅立叶变换回旋共振质谱（FTICR MS）和* H NMR以及CHNOS和尺寸排阻色谱法研究Trametes sp对HA降解的机理。 M23和Phaner-ochaete sp。 Y6。尺寸排阻色谱分析证明并提供了HA分解为低分子量化合物的证据。 * H NMR分析显示出氧化，芳族含量的减少以及在生物降解过程中HA脱甲基的迹象。还使用CHNOS获得了氧化证据。使用我们小组开发的新软件程序（David Mass Sort）对FTICR MS结果进行分析，发现连续的一系列质量表明生物化学降解趋势，例如氧化，芳族裂解和去甲基化。这些结果与1 H NMR分析以及导致HA降解的水溶酶系统的建议作用相吻合。

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  • 来源
    《Environmental Science & Technology》 |2011年第7期|p.2748-2754|共7页
  • 作者

    Tzafrir Grinhut; Norbert Hertkorn; Philippe Schmitt-Kopplin; Yitzhak Hadar; Yona Chen;

  • 作者单位

    The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100,Israel;

    HelmhoItz Zentrum Miinchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

    HelmhoItz Zentrum Miinchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Ingolstadter Landstr. 1, 85764 Neuherberg, Germany;

    The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100,Israel;

    The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100,Israel;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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