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首页> 外文期刊>Environmental Science & Technology >Isolation and Identification of Ligands for the Goldfish Testis Androgen Receptor in Chemical Recovery Condensates from a Canadian Bleached Kraft Pulp and Paper Mill
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Isolation and Identification of Ligands for the Goldfish Testis Androgen Receptor in Chemical Recovery Condensates from a Canadian Bleached Kraft Pulp and Paper Mill

机译：加拿大漂白牛皮纸浆造纸厂化学回收冷凝物中金鱼睾丸雄激素受体的配体的分离与鉴定

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摘要

This study builds on a series of investigations characterizing substances in kraft mill chemical recovery condensates that depress sex steroids in fish. Here, incubations of goldfish testis androgen receptors (AR) with condensate extracts were used to investigate the potential role of androgens in hormone depressions. Condensates contained variable levels of AR ligands, with the highest amounts in nonpolar extracts of filtered solids prior to solid phase extraction (SPE). High pressure liquid chroma- tography (HPLC) fractionatjon recovered the majority of activity in one fraction, with ligands detected in three additional fractions. Gas chroma- tography mass spectrometry analysis of the most active fraction confirmed the two most abundant components as the diterpenes manool and geranyl linalooL Manool exhibited a relative affinity for the AR that was 300 fold less than testosterone and accounted for 26% of total filtered solids activity. Geranyl linalool exhibited no affinity for the AR. Three additional diterpenoid families were tentatively identified as principal components of the three other androgenic HPLC fractions. Compared to condensates, final effluent had 3000 fold less androgenic activity, with <1% attributable to manool. Putative androgens previously associated with mill effluents (androstenedione and androstadienedione) and progesterone were not detected; however, additional condensate diterpenes suspected as androgens were identified in final effluent. This study is the first to confirm nonsteroidal cyclic diterpenes as androgenk at pulp mills. A major in-mill source of these substances was identified, and the role of androgens in mill effluents affecting fish reproduction was reinforced.
机译：这项研究建立在一系列研究的基础上，这些研究表征了牛皮纸厂化学回收冷凝物中的物质，这些物质可抑制鱼类中的性类固醇。在这里，金鱼睾丸雄激素受体（AR）与凝析液的孵育用于研究雄激素在激素抑制中的潜在作用。冷凝物包含可变含量的AR配体，在固相萃取（SPE）之前，非极性萃取物的过滤固体含量最高。高压液相色谱（HPLC）馏分在一个馏分中回收了大部分活性，在另外三个馏分中检测到了配体。气相色谱质谱法分析活性最高的馏分，确认了两个最丰富的成分，因为二萜烯醇和香叶基芳樟醇对AR的相对亲和力比睾丸激素低300倍，占总过滤固体活性的26％ 。香叶基芳樟醇对AR没有亲和力。暂定将另外三个二萜类家族确定为其他三个雄激素HPLC组分的主要成分。与冷凝液相比，最终流出物的雄激素活性降低了3000倍，其中<1％可归因于Manol。未检测到先前与工厂废水（雄烯二酮和雄二烯二酮）和孕酮相关的假定雄激素；但是，在最终出水中还发现了疑似雄激素的其他冷凝物二萜。这项研究是第一个在纸浆厂确定非甾体环状二萜为雄激素的药物。确定了这些物质在厂内的主要来源，并加强了雄激素在影响鱼类繁殖的厂内废水中的作用。

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  • 来源
    《Environmental Science & Technology》 |2011年第23期|p.10226-10234|共9页
  • 作者

    Philip D. Scott; Craig B. Milestone; D. Scott Smith; Deborah L. MacLatchy; L. Mark Hewitt;

  • 作者单位

    Wilfrid Laurier University, Waterloo, Ontario, Canada;

    Wilfrid Laurier University, Waterloo, Ontario, Canada,Environment Canada, Burlington, Ontario, Canada;

    Wilfrid Laurier University, Waterloo, Ontario, Canada;

    Wilfrid Laurier University, Waterloo, Ontario, Canada;

    Environment Canada, Burlington, Ontario, Canada,National Water Research Institute, Environment Canada, 867 Lakeshore Road, Burlington, ON, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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