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首页> 外文期刊>Environmental Science & Technology >Disruption of the Steroid Metabolome in Fish Caused by Exposure to the Environmental Estrogen 17α-Ethinylestradiol
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Disruption of the Steroid Metabolome in Fish Caused by Exposure to the Environmental Estrogen 17α-Ethinylestradiol

机译：暴露于环境雌激素17α-乙炔雌二醇引起的鱼类类固醇代谢紊乱

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摘要

Exposure to environmental estrogens such as 17a-ethinylestradiol (EE2) has been associated with feminization and a decline in fertility of male fish. To investigate the effect of estrogen exposure on steroid homeostasis, we exposed roach (Rutilus rutilus) to EE2 (1-29 ng/L) for 18 days and analyzed steroid profiles in bile and plasma using targeted analyses and in liver and gonadal tissues using mass spectrometry metabolite profiling techniques (metabolomics). Exposure to EE2 resulted in a concentration dependent reduction of estrogens and androgens in bile and plasma of both male and female fish. At 10 ngEE2/L, significant reductions in concentrations of hydroxyprogesterone, androstenedione, 11-hydroxyandrostene-dione, and 11-ketotestosterone were detected in the testes metabolome, indicating disruption of steroid biosynthesis upstream of androgen metabolism. Estrogen exposure also resulted in increased biosynthesis of cortisol and cortisone in testes and ovaries, respectively, but did not alter glucocorticoid concentrations in the liver or plasma. This first report on the effect of EE2 exposure on the steroid metabolome in fish tissues suggests that both sex steroid and glucocorticoid pathways are one of the primary targets of estrogen exposure in fish gonads and provides further insights into the mode of action of this endocrine disrupting chemical.
机译：暴露于环境雌激素（例如17a-炔雌醇（EE2））与女性化和公鱼育性下降有关。为了研究雌激素暴露对类固醇稳态的影响，我们将蟑螂（Rutilus rut​​ilus）暴露于EE2（1-29 ng / L）达18天，并使用靶向分析法分析了胆汁和血浆中的类固醇谱，并使用质量分析了肝脏和性腺组织中光谱分析代谢物谱分析技术（代谢组学）。暴露于EE2导致雄性和雌性鱼类胆汁和血浆中雌激素和雄激素的浓度依赖性降低。在10 ngEE2 / L时，睾丸代谢组中的羟基孕酮，雄烯二酮，11-羟基雄烯二酮和11-酮睾酮的浓度显着降低，这表明雄激素代谢上游甾体生物合成受到破坏。雌激素暴露还分别导致睾丸和卵巢中皮质醇和可的松的生物合成增加，但并未改变肝脏或血浆中糖皮质激素的浓度。关于EE2暴露对鱼组织中类固醇代谢组的影响的第一份报告表明，性类固醇和糖皮质激素途径都是鱼性腺中雌激素暴露的主要目标之一，并提供了对该内分泌干扰物化学作用方式的进一步见解。 。

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  • 来源
    《Environmental Science & Technology》 |2010年第9期|P.3552-3558|共7页
  • 作者

    ANEL M. FLORES-VALVERDE; rnJULIA HORWOOD; rnELIZABETH M. HILL;

  • 作者单位

    School of Life Sciences, University of Sussex, Falmer, Brighton, U.K. BN1 9QG;

    rnSchool of Life Sciences, University of Sussex, Falmer, Brighton, U.K. BN1 9QG;

    rnSchool of Life Sciences, University of Sussex, Falmer, Brighton, U.K. BN1 9QG;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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