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首页> 外文期刊>Environmental Science & Technology >Effects of Solvents and Dosing Procedure on Chemical Toxicity in Cell-Based in Vitro Assays
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Effects of Solvents and Dosing Procedure on Chemical Toxicity in Cell-Based in Vitro Assays

机译：溶剂和加药程序对基于细胞的体外化学毒性的影响

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摘要

Due to the implementation of new legislation, such as REACh, a dramatic increase of animal use for toxicity testing is expected and the search for alternatives is timely. Cell-based in vitro assays are promising alternatives. However, the behavior of chemicals in these assays is still poorly understood. We set out to quantify the exposure and associated toxicity of chemicals with different physicochemical properties toward a fish gill cell line when different solvents and procedural steps are used to introduce test chemicals to cells. Three chemicals with a range ol hydrophobicity and volatility were selected and delivered in three different solvents using two common dosing procedures. Toxicity tests were coupled with chemical analysis to quantify the chemical concentrations within culture wells. The impact of solvents and dosing procedure was greatest for the most volatile and hydrophobic test chemical. We show that certain combinations of the test chemical, solvent, and procedural steps can lead to inhomogeneous distribution of the test chemical and thus differing degrees of bioavailability, resulting in quantitative differences in apparent toxicity.
机译：由于实施了诸如REACh之类的新法规，预计动物在毒性试验中的使用将急剧增加，因此寻找替代品是及时的。基于细胞的体外测定是有前途的替代方法。但是，对于这些测定中化学物质的行为仍知之甚少。我们着手量化当使用不同的溶剂和程序步骤将测试化学物质引入细胞时，具有不同物理化学性质的化学物质对鱼g细胞系的暴露和相关毒性。选择了三种具有一定疏水性和挥发性的化学品，并使用两种常见的加料程序将其输送到三种不同的溶剂中。毒性测试与化学分析相结合以量化培养孔内的化学浓度。对于最具挥发性和疏水性的测试化学品，溶剂和加药程序的影响最大。我们表明，测试化学药品，溶剂和程序步骤的某些组合会导致测试化学药品的分布不均匀，从而导致不同的生物利用度，从而导致表观毒性的定量差异。

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  • 来源
    《Environmental Science & Technology》 |2010年第12期|P.4775-4781|共7页
  • 作者

    KATRIN TANNEBERGER; rnANGELES RICO-RICO; rnNYNKE I. KRAMER; rnFRANS J. M. BUSSER; rnJOOP L. M. HERMENS; rnKRISTIN SCHIRMER;

  • 作者单位

    Eawag, Swiss Federal Institute of Aquatic Science and Technology, UEberlandstrasse 133, 8600 Duebendorf, Switzerland;

    rnInstitute for Risk Assessment Sciences, University of Utrecht, Yalelaan 2, 3584 CM Utrecht, The Netherlands INIA-Departmem of Environment, Ctra de la Coruna, Km 7,28040 Madrid, Spain;

    rnInstitute for Risk Assessment Sciences, University of Utrecht, Yalelaan 2, 3584 CM Utrecht, The Netherlands;

    rnInstitute for Risk Assessment Sciences, University of Utrecht, Yalelaan 2, 3584 CM Utrecht, The Netherlands;

    rnInstitute for Risk Assessment Sciences, University of Utrecht, Yalelaan 2, 3584 CM Utrecht, The Netherlands;

    rnEawag, Swiss Federal Institute of Aquatic Science and Technology, UEberlandstrasse 133, 8600 Duebendorf, Switzerland ETH Zuerich, Institute of Biogeochemistry and Pollutant Dynamics, Universitaetstrasse 16, 8092 Zuerich, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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