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首页> 外文期刊>Environmental Science & Technology >Environmental Reservoirs for Enterotoxigenic Escherichia coli in South Asian Gangetic Riverine System
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Environmental Reservoirs for Enterotoxigenic Escherichia coli in South Asian Gangetic Riverine System

机译：南亚恒河河流系统中产肠毒素大肠杆菌的环境储层

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摘要

Forecasting diarrheagenic E. coli contamination of aquatic resources to prevent outbreaks largely depends on rapid and accurate diagnostic testing in a few hours. Real-time PCR is widely used for quick culture-free quantitative enumeration of pathogenic bacteria in environmental samples. In this study, real-time PCR in molecular beacon format was used for detection and culture-free quantitative enumeration of enterotoxigenic Escherichia coli(ETEC) harboring LT1 gene in a sewage-impacted south Asian Gangetic riverine system. The quantitative budget for ETEC in surface water was observed to vary significantly (DMRT, p < 0.05) among the sites. Aquatic flora (Eichhornia crassipes, Potamogeton crispus, Pota-mogeton pectinatus, Ranunculus sceleratus, Polygonum glabrum, Pontederia cordata, Najas Mica and strands of Spirogyra spp.) collected between sites 1 and 9 exhibited significant high levels of ETEC in comparison to their representatives collected from pristine area. The level of ETEC harboring LT1 gene observed in leafy vegetables cultivated along the banks was in the following order mint leaves > coriander > spinach > methi leaves. The study suggests that the aquatic flora and cultivated leafy vegetables in the south Asian Gangetic riverine system are environmental reservoirs for enterotoxigenic Escherichia coli.
机译：预测腹泻性大肠杆菌对水生资源的污染以防止疾病爆发在很大程度上取决于在数小时内进行的快速准确的诊断测试。实时荧光定量PCR广泛用于环境样品中病原菌的快速无培养定量计数。在这项研究中，以分子信标格式的实时PCR用于检测和无细菌定量枚举污水影响南亚Gangetic河流系统中含有LT1基因的肠毒素性大肠杆菌（ETEC）。观测到的ETEC在地表水中的定量预算在各地点之间存在显着差异（DMRT，p <0.05）。在站点1和9之间收集的水生植物群（凤眼棘鱼，Potamogeton crispus，Potamogeton pectinatus，毛an毛，何首乌，蓬头草，Najas云母和螺旋藻属）在与它们所收集的代表相比具有较高的ETEC水平来自原始地区。在沿岸种植的带叶蔬菜中观察到的带有LT1基因的ETEC的水平按以下顺序排列：薄荷叶>香菜>菠菜> methi叶。研究表明，南亚恒河河流系统中的水生植物和种植的带叶蔬菜是产肠毒素大肠杆菌的环境储藏室。

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  • 来源
    《Environmental Science & Technology》 |2010年第16期|p.6475-6480|共6页
  • 作者

    GULSHAN SINGH; POORNIMA VAJPAYEE; SIYA RAM; RISHI SHANKER;

  • 作者单位

    Indian Institute of Toxicology Research (C.S.I.R.), Post Box No.80, Manama Gandhi Marg Lucknow -226001, U.P., India;

    rnIndian Institute of Toxicology Research (C.S.I.R.), Post Box No.80, Manama Gandhi Marg Lucknow -226001, U.P., India;

    rnIndian Institute of Toxicology Research (C.S.I.R.), Post Box No.80, Manama Gandhi Marg Lucknow -226001, U.P., India;

    rnIndian Institute of Toxicology Research (C.S.I.R.), Post Box No.80, Manama Gandhi Marg Lucknow -226001, U.P., India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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