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Method for the Analysis of Oxygen Isotopic Composition of Soil Phosphate Fractions

机译：土壤磷酸盐组分中氧同位素组成的分析方法

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摘要

The isotopic signature of oxygen in phosphate (δ~(18)O_p) of various soil fractions may shed light on P transformations, including phosphorus (P) recycling by soil microorganisms, uptake by plants and P adsorption, precipitation and release by oxides and minerals, thus increasing our understanding on P cycling and lability in soils. We developed and tested a protocol to extract and purify inorganic phosphate (Pi) from different soil fractions distinguished by binding strength and precipitate it as silver phosphate (Ag_3PO_4) for <δ~(18)O_p analysis. Soil P is extracted sequentially using water, NaHCOi NaOH and HCI and Pi in each solution is purified and precipitated as Ag_3PO_4 The unique characteristics and possible interferences of the soil solution extracts are addressed. Two agricultural soil samples receiving reclaimed wastewater or fresh water were analyzed, and results indicate that all soil fractions analyzed have been impacted to some degree by biologically enzyme mediated cycling of P in the soil.
机译：各种土壤组分的磷酸盐（δ〜（18）O_p）中氧的同位素特征可能揭示了磷的转化，包括土壤微生物对磷（P）的循环利用，植物的吸收和磷的吸收，氧化物和矿物质的沉淀和释放，从而增加了我们对土壤中磷循环和不稳定性的理解。我们开发并测试了从具有结合强度的不同土壤部分提取和纯化无机磷酸盐（Pi）并将其沉淀为磷酸银（Ag_3PO_4）的方案，用于<δ〜（18）O_p分析。依次用水，NaHCOi NaOH和HCl和Pi萃取土壤P，然后纯化并以Ag_3PO_4的形式沉淀。研究了土壤溶液萃取物的独特特性和可能的​​干扰。对接受再生废水或淡水的两个农业土壤样品进行了分析，结果表明，所分析的所有土壤组分均受到生物酶介导的土壤中P循环的一定程度的影响。

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  • 来源
    《Environmental Science & Technology》 |2010年第19期|p.7583-7588|共6页
  • 作者

    IRIS ZOHAR; AVI SHAVIV; TATANIA KLASS; KATHRYN ROBERTS; ADINA PAYTAN;

  • 作者单位

    Department of Environmental, Water and Agricultural Engineering, Faculty of Civil and Environmental Engineering,The Technion-Israel Institute of Technology, Haifa, Israel 32000;

    rnDepartment of Environmental, Water and Agricultural Engineering, Faculty of Civil and Environmental Engineering,The Technion-Israel Institute of Technology, Haifa, Israel 32000;

    rnInstitute of Marine Sciences, University of California Santa Cruz., 1156 High St., Santa Cruz. California 95064;

    rnInstitute of Marine Sciences, University of California Santa Cruz., 1156 High St., Santa Cruz. California 95064;

    rnInstitute of Marine Sciences, University of California Santa Cruz., 1156 High St., Santa Cruz. California 95064;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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