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首页> 外文期刊>Environmental Science & Technology >Dioxin-like Activity in Japanese Indoor Dusts Evaluated by Means of in Vitro Bioassay and Instrumental Analysis: Brominated Dibenzofurans Are an Important Contributor
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Dioxin-like Activity in Japanese Indoor Dusts Evaluated by Means of in Vitro Bioassay and Instrumental Analysis: Brominated Dibenzofurans Are an Important Contributor

机译：通过室内生物测定和仪器分析对日本室内粉尘中的二恶英类活性进行评估：溴化二苯并呋喃是重要的贡献者

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摘要

In our previous study, we demonstrated that dioxin-like activity in suKuric-acid-treated extracts of Japanese indoor dust was higher than the activity in contaminated sediments. In the current study, we used high-performance liquid chromatography (HPLC) fracuonation in combination with the dioxin-responsive chemical-activated luciferase gene expression (DR-CALUX) assay and gas chromatography-high-resolurjon mass spectrometry (GC-HRMS) to quantitatively evaluate dioxin-like compounds in indoor dusts. Selected four suKuric-acid-treated extracts of indoor dusts were fractionated into seven fractions by means of HPLC with a nitrophenylpropylsilica column, and the activity in the first fraction of each extract was much higher than the activities in the other fractions. Therefore, each of the first fractions was further fractionated into 90 fractions by HPLC with an octadecylsilica column, and all the fractions were analyzed by means of the DR-CALUX assay. On the basis of eluton characteristics, the active compounds in the fractions showing high activity were determined to be polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/ Fs), coplanar polychlorinated biphenyls (Co-PCBs), and polychlorinated naphthalenes (PCNsi, or supposed to be polybrominated dibenzo-p-dioxins and dibenzofurans (PB0D7 Fs). These compounds in the 33 dusts extracts were quantified by means of GC-HRMS, and their median-based contributions of the theoretical CALUX-TEQs [2,3,7.8-tetrachlorodibenzo-p-dioxin (2,3,7,8-tetraCDD) equivalent) chemically calculated for PBDFs, PCDOs, PCDFs, non-ortho Co-PCBs, PCNs, and mono-ortho Co-PCBs to the experimental values (38-1400 pg/g, median 160 pg/g) were 17%, 14%, 8.8%, 0,98%, 0.10%, and 0.019%, respectively. Our results indicate that PBDFs, which are not internationally regulated dioxins, were important contributors to dioxin-like activity in Japanese dust and that further analysis of PBDFs in indoor dust is required so that the risk of exposure to these compounds via dust can be assessed.
机译：在我们先前的研究中，我们证明了日本室内粉尘的经硫酸处理的二恶英类活性高于受污染的沉积物中的二恶英类活性。在本研究中，我们将高效液相色谱（HPLC）裂解与二恶英反应性化学激活的荧光素酶基因表达（DR-CALUX）分析和气相色谱-高分辨质谱（GC-HRMS）结合使用定量评估室内灰尘中的二恶英类化合物。用硝基苯丙基硅胶柱通过HPLC将选定的四种经苏酸处理的室内粉尘提取物分为7个馏分，每个提取物的第一馏分的活性远高于其他馏分的活性。因此，使用十八烷基硅胶柱通过HPLC进一步将每个第一级分分离成90个级分，并且通过DR-CALUX测定法分析所有级分。根据洗脱液的特性，将显示出高活性的馏分中的活性化合物确定为多氯二苯并对二恶英和二苯并呋喃（PCDD / Fs），共面多氯联苯（Co-PCBs）和多氯萘（PCNsi，或33种粉尘提取物中的这些化合物通过GC-HRMS定量，并基于理论CALUX-TEQ的中位贡献[2,3,7.8化学计算得出的PBDF，PCDO，PCDF，非邻位Co-PCB，PCN和单邻位Co-PCB的-四氯二苯并-对二恶英（2,3,7,8-tetraCDD）当量为实验值（38 -1400 pg / g，中位数160 pg / g）分别为17％，14％，8.8％，0.98％，0.10％和0.019％。我们的结果表明，不是国际管制的二恶英的PBDF是日本粉尘中二恶英样活性的重要贡献者，需要对室内粉尘中的PBDF进行进一步分析，以便评估通过粉尘暴露于这些化合物的风险。

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  • 来源
    《Environmental Science & Technology》 |2010年第21期|p.8330-8336|共7页
  • 作者

    GO SUZUKI; MASAYUKI SOMEYA; SHIN TAKAHASHI; SH1NSUKE TANABE; SHIN-ICHI SAKAI; HIDETAKA TAKIGAMI;

  • 作者单位

    Research Center for Material Cycles and Waste Management,National Institute for Environmental Studies Tsukuba 305-8506, Japan,Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    rnCenter for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    rnCenter for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    rnCenter for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;

    rnEnvironment Preservation Center, Kyoto University,Kyoto 606-8501, Japan;

    rnResearch Center for Material Cycles and Waste Management,National Institute for Environmental Studies Tsukuba 305-8506, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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