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首页> 外文期刊>Environmental Science & Technology >Denitration Of 2,4,6-trinitrotoluene In Aqueous Solutions Using Small-molecular-weight Catalyst(s) Secreted By Pseudomonas Aeruginosa Esa-5
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Denitration Of 2,4,6-trinitrotoluene In Aqueous Solutions Using Small-molecular-weight Catalyst(s) Secreted By Pseudomonas Aeruginosa Esa-5

机译：铜绿假单胞菌Esa-5分泌的小分子量催化剂在水溶液中脱硝2,4,6-三硝基甲苯

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摘要

The denitration of 2,4,6-trinitrotoluene (TNT) can produce mono- or dinitro aromatic compounds susceptible to microbial mineralization. In the present study, denitration of TNT and other nitro aromatic compounds was investigated with a solid-phase extract obtained from the culture supernatant of Pseudomonas aeruginosa ESA-5 grown on a chemically defined aerobic medium. When the C_(18) solid-phase extract containing extracellular catalysts (EC) was incubated with TNT and NAD(P)H, we observed a significant release of nitrite. The concentration of nitrite released in the reaction medium was strongly dependent on the concentration of NAD(P)H and EC. Denitration also occurred with two TNT-related molecules, 2,4,6-trinitrobenzaldehyde, and 2,4,6-trinitrobenzyl alcohol. The release of nitrite was coupled with the formation of two polar metabolites, and mass spectrometry analyses indicated that each of these compounds had lost two nitro groups from the trinitro aromatic parent molecule. During this process, the production of toxic reduced TNT metabolites was minimal. The incubation of EC with TNT, NAD(P)H, and specific scavengers of reactive oxygen species suggested the involvement of superoxide radicals (O_2~-) and hydrogen peroxide in the denitration process. Results obtained in this study reveal for the first time that extracellular small-molecular-weight substance(s) of bacterial origin can serve as green catalyst(s) to initiate TNT denitration. In addition, this study gives clear evidence for the production of a TNT metabolite bearing a single nitro group following a denitration reaction with catalyst(s) of biotic origin.
机译：2,4,6-三硝基甲苯（TNT）的脱硝作用会产生易于被微生物矿化的单或二硝基芳族化合物。在本研究中，使用固相萃取物研究了TNT和其他硝基芳族化合物的脱硝作用，该固相萃取物是从生长在化学需氧培养基上的铜绿假单胞菌ESA-5的培养上清液获得的。当将含有细胞外催化剂（EC）的C_（18）固相萃取物与TNT和NAD（P）H孵育时，我们观察到亚硝酸盐的大量释放。反应介质中释放的亚硝酸盐浓度强烈取决于NAD（P）H和EC的浓度。两个TNT相关分子，2,4,6-三硝基苯甲醛和2,4,6-三硝基苄醇也发生了反硝化作用。亚硝酸盐的释放与两种极性代谢物的形成有关，质谱分析表明，这些化合物各自从三硝基芳族母体分子中丢失了两个硝基。在此过程中，毒性降低的TNT代谢产物的产生极少。 EC与TNT，NAD（P）H和特定的活性氧清除剂的孵育表明，超氧自由基（O_2〜-）和过氧化氢参与了脱硝过程。这项研究获得的结果首次揭示了细菌来源的细胞外小分子物质可以作为绿色催化剂来引发TNT脱硝。此外，这项研究为与生物来源的催化剂进行脱硝反应后产生带有单个硝基的TNT代谢产物提供了明确的证据。

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