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首页> 外文期刊>Environmental Science & Technology >Airborne Virus Capture and Inactivation by an Electrostatic Particle Collector
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Airborne Virus Capture and Inactivation by an Electrostatic Particle Collector

机译：静电粒子收集器捕获机载病毒并使其灭活

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摘要

Airborne virus capture and inactivation were studied in an electrostatic precipitator (ESP) at applied voltages from -10 to +10 kV using aerosolized bacteriophages T3 and MS2. For each charging scenario, samples were collected from the effluent air stream and assayed for viable phages using plaque assays and for nucleic acids using quantitative polymerase chain reaction (qPCR) assays. At higher applied voltages, more virus particles were captured from air with maximum log reductions of 6.8 and 6.3 for the plaque assay and 4.2 and 3.5 for the qPCR assay at -10 kV for T3 and MS2, respectively. Beyond corona inception (i.e., at applied voltages of -10, -8, +8, and +10 kV), log reduction values obtained with the plaque assay were much higher compared to those of the qPCR assay because nonviable particles, while present in the effluent were unaccounted forinthe plaque assay. Comparisons of these assays showed that in-flight inactivation (i.e., inactivation without capture) was greater for the highest applied voltages with a log inactivation of 2.6 for both phages at -10 kV. We have demonstrated great potential for virus capture and inactivation via continual ion and reactive species bombardment when conditions in the ESP are enforced to generate a corona discharge.
机译：使用雾化的噬菌体T3和MS2在静电除尘器（ESP）中在施加电压-10至+10 kV的条件下研究了机载病毒的捕获和灭活。对于每种充电方案，从流出的气流中收集样品，并使用噬菌斑测定法检测活噬菌体，并使用定量聚合酶链反应（qPCR）测定法测定核酸。在更高的施加电压下，从空气中捕获到更多的病毒颗粒，对于噬菌斑测定，在10 kV时，T3和MS2的最大对数减少分别为6.8和6.3，qPCR测定的对数最大减少为4.2和3.5。除了电晕开始（即，在施加的电压分别为-10，-8，+ 8和+10 kV时）之外，与非PCR试剂盒相比，通过噬菌斑检测仪获得的对数减少值要高得多，因为无活力粒子虽然存在于在噬菌斑测定中，流出物未说明。这些测定法的比较表明，对于最高施加电压，飞行中的失活（即，没有捕获的失活）更大，在-10kV下两个噬菌体的对数失活为2.6。当强制执行ESP中的条件以产生电晕放电时，我们证明了通过连续的离子和反应性物质轰击病毒捕获和灭活的巨大潜力。

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  • 来源
    《Environmental Science & Technology》 |2009年第15期|5940-5946|共7页
  • 作者

    ERIC M. KETTLESON; BALA RAMASWAMI; CHRISTOPHER J. HOGAN JR.; MYONG-HWA LEE; GENNADIY A. STATYUKHA; PRATIM BISWAS; LARGUS T. ANGENENT;

  • 作者单位

    Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130;

    Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130;

    Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130;

    Environmental and Energy Division, Korea Institute of Industrial Technology, CheonAn City, South Korea;

    Cybernetics of Chemical Technology Processes, National Technical University of Ukraine, Kiev, Ukraine;

    Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130;

    Department of Biological and Environmental Engineering, Cornell University, 214 Riley-Robb Hall, Ithaca, New York 14853;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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