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Magnetic Enzyme Carrier for Effective Biofouling Control in the Membrane Bioreactor Based on Enzymatic Quorum Quenching

机译:基于酶法定量猝灭的膜生物反应器中有效控制生物结垢的磁性酶载体

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摘要

Quorum quenching, enzymatic quenching of bacterial quorum sensing, has recently proven its potential as a novel approach of biofouling control in the membrane bioreactor (MBR) for advanced wastewater treatment However, the short catalytic lifetime and difficulty in recovering free enzyme hamper the successful application of the quorum quenching technique in the MBR under a long-term continuous operation. In this study, a magnetic enzyme carrier (MEC) was prepared by immobilizing the quorum quenching enzyme (acylase) on magnetic particles to overcome the technical limitations of free enzyme. The MEC showed no activity decrease under both continuous shaking for 14 days and 29 iterative cycles of reuse. Furthermore, the comparison of the MEC with free enzyme in a batch type MBR showed that the MEC efficiently alleviated the membrane biofouling and showed a great advantage over free enzyme in terms of recycled use and stability in mixed liquor. When the MEC was applied to the lab scale MBR in a continuous operation, it also enhanced the membrane permeability to a large extent compared with a conventional MBR with no enzyme.
机译:群体猝灭(细菌群体感应的酶促猝灭)最近已证明其潜力,可作为膜生物反应器(MBR)中用于高级废水处理的新型生物污垢控制方法。长期连续运行下MBR的定额淬火技术。在这项研究中,通过将群体猝灭酶(酰基转移酶)固定在磁性颗粒上来制备磁性酶载体(MEC),以克服游离酶的技术局限性。在连续摇动14天和29个重复使用循环下,MEC均未显示活性降低。此外,在间歇式MBR中将MEC与游离酶进行比较表明,MEC有效地减轻了膜的生物结垢,并且在循环利用和混合液稳定性方面显示出优于游离酶的巨大优势。当MEC以连续操作方式应用于实验室规模的MBR时,与没有酶的常规MBR相比,它在很大程度上也提高了膜的渗透性。

著录项

  • 来源
    《Environmental Science & Technology》 |2009年第19期|7403-7409|共7页
  • 作者单位

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    Department of Chemical and Biological Engineering, Korea University,Seoul, 136-701, Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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