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首页> 外文期刊>Environmental Science & Technology >Role of Interfacial Tensions in the Translocation of Rhodococcus erythropolis during Growth in a Two Phase Culture
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Role of Interfacial Tensions in the Translocation of Rhodococcus erythropolis during Growth in a Two Phase Culture

机译:两相培养过程中界面张力在红球红球菌转运中的作用

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摘要

Rhodococcus erythropolis PR4 is an alkane-degrading bacterium, which grows well in media containing high concentrations of alkanes. These properties give the organism potential in the bioremediation of various environments contaminated by alkanes. In this study, we report the translocation of R. erythropolis PR4 from an aqueous phase to an alkane phase during growth in a two phase culture medium. When the alkane chain length was between C10 and C12, PR4 was located at the aqueous-alkane interface, but when the alkane chain length was above C14, PR4 translocated into the alkane phase. Complete translocation into alkane phase was accompanied by normal growth, whereas interfacial localization hampered growth, indicating that localization among other possible factors, play an important role in the growth of R. erythropolis PR4 in two phase cultures. The PR4 cell surface was physico-chemically characterized in terms of its cell surface charge and surface free energy. Contact angles were measured on bacterial lawns, followed by thermodynamic analyses of Gibbs free energies for localization of PR4 in the aqueous or alkane phase or at the interface. Although entry into the alkane phase of PR4 grown in the presence of both C12 and C19 was thermodynamically favorable, translocation from the inside of the alkane phase to the interface was only favorable for PR4 grown in the presence of C12. In line with these thermodynamic analyses, two phase partitioning showed that PR4 grown in the presence of C12 and C19 were more hydrophobic than PR4 grown in the presence of lower alkanes, while C12 grown bacteria were less lipophilic than C19 grown bacteria. In conclusion, the localization of R. erythropolis PR4 in a two phase culture medium is thermodynamically driven to facilitate its optimal growth.
机译:红球红球菌PR4是一种降解烷烃的细菌,在含有高浓度烷烃的培养基中生长良好。这些特性使生物体具有在被烷烃污染的各种环境中进行生物修复的潜力。在这项研究中,我们报告了在两相培养基中生长过程中红细菌RPR4从水相到烷烃相的易位。当烷烃链长度在C10和C12之间时,PR4位于含水烷烃界面处,但是当烷烃链长度在C14以上时,PR4易位到烷烃相中。完全易位进入烷烃相伴随正常生长,而界面本地化阻碍了生长,这表明本地化以及其他可能的因素在两相培养中对R. erythropolis PR4的生长起着重要作用。 PR4细胞表面根据其细胞表面电荷和表面自由能进行了物理化学表征。在细菌草坪上测量接触角,然后对吉布斯自由能进行热力学分析,以将PR4定位在水相或烷烃相中或界面处。尽管在C12和C19均存在的情况下进入生长的PR4的烷烃相在热力学上是有利的,但是从烷烃相内部到界面的转移仅对在C12存在下生长的PR4是有利的。与这些热力学分析一致,两相分配显示在C12和C19存在下生长的PR4比在低级烷烃存在下生长的PR4更疏水,而C12生长的细菌比C19生长的细菌更不亲脂。总而言之,热力学上R. erythropolis PR4的定位是由热力学驱动的,以促进其最佳生长。

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  • 来源
    《Environmental Science & Technology》 |2009年第21期|8290-8294|共5页
  • 作者单位

    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, PO Box 196, Groningen, 9700AD, The Netherlands;

    Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, PO Box 196, Groningen, 9700AD, The Netherlands;

    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan;

    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan;

    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan;

    Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, PO Box 196, Groningen, 9700AD, The Netherlands;

    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan;

    Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, PO Box 196, Groningen, 9700AD, The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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