...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Role off Pseudomonas aeruginosa Biofilm in the Initial Adhesion, Growth and Detachment of Escherichia coli in Porous Media
【24h】

Role off Pseudomonas aeruginosa Biofilm in the Initial Adhesion, Growth and Detachment of Escherichia coli in Porous Media

机译：铜绿假单胞菌生物膜在多孔介质中大肠杆菌的初始黏附，生长和分离中的作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study systematically investigated the impact of Pseudomonas aeruginosa biofilm on the initial adhesion, growth, and detachment of indicator bacteria Escherichia coli JM109 in porous media. Two P. aeruginosa strains, the mucoid PDO300 and wide type PA01 with different extracellular polymeric substance (EPS) composition and secretion capability, were used to grow biofilm in packed beds. Results from the column breakthrough curves and retained JM109 profiles show that the amount and composition of P. aeruginosa biofilm EPS have a profound impact on the deposition and retention of f. coli in porous media. PA01 biofilm coating improved E. coli retention in the column, whereas PD0300 biofilm coating had only a small impact on E. coli removal. Biofilm surface hydrophobicity and polymeric interactions between the biofilm and E. coli cell surfaces were found to play important roles in controlling the distribution of E. coli along the columns. After initial attachment, E. coli bacteria were able to survive and grow at similar growth rates in columns coated with either PA01 or PD0300 biofilms with a relatively low nutrient supply. Biofilm detachment was the major mechanism that introduced E. coli bacteria to the bulk fluid long after the contamination event when E. coli cells became an integral part of the biofilm. Findings of this study suggest that biofilm plays a significant role in controlling the initial attachment, growth, and survival of bacteria in porous media, and that the interaction between bacteria and biofilm surfaces should be considered when predicting bacterial and pathogen migration in the environment.
机译：这项研究系统地研究了铜绿假单胞菌生物膜对多孔介质中指示菌大肠杆菌JM109的初始黏附，生长和脱离的影响。两种铜绿假单胞菌菌株，具有不同细胞外聚合物（EPS）组成和分泌能力的粘液状PDO300和宽型PA01，用于在填充床上生长生物膜。柱穿透曲线和保留的JM109谱图的结果表明，铜绿假单胞菌生物膜EPS的数量和组成对f的沉积和保留有深远的影响。多孔介质中的大肠杆菌。 PA01生物膜涂层改善了大肠杆菌在色谱柱中的保留，而PD0300生物膜涂层对去除大肠杆菌的影响很小。发现生物膜表面疏水性和生物膜与大肠杆菌细胞表面之间的聚合物相互作用在控制大肠杆菌沿色谱柱的分布中起重要作用。最初附着后，大肠杆菌细菌能够在以相对较低养分供应的PA01或PD0300生物膜包被的柱子中存活并以相似的增长率生长。生物膜分离是在污染事件发生很长时间之后，即大肠杆菌细胞成为生物膜的组成部分时，才将大肠杆菌细菌引入体液的主要机制。这项研究的发现表明，生物膜在控制细菌在多孔介质中的初始附着，生长和存活中起着重要作用，并且在预测环境中细菌和病原体的迁移时应考虑细菌与生物膜表面之间的相互作用。

著录项

  • 来源
    《Environmental Science & Technology》 |2008年第2期|443-449|共页
  • 作者

    YANG LIU; JIN LI;

  • 作者单位
  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号