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首页> 外文期刊>Environmental Science & Technology >Carbon Nanotube-Based Electrochemical Sensor for Assay of Salivary Cholinesterase Enzyme Activity: An Exposure Biomarker of Organophosphate Pesticides and Nerve Agents
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Carbon Nanotube-Based Electrochemical Sensor for Assay of Salivary Cholinesterase Enzyme Activity: An Exposure Biomarker of Organophosphate Pesticides and Nerve Agents

机译：碳纳米管的唾液胆碱酯酶活性测定的电化学传感器：有机磷酸酯农药和神经制剂的暴露生物标志物。

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摘要

Certain saliva enzymes may be useful biomarkers for detecting exposures to organophosphate pesticides and chemical nerve agents. In this regard, saliva biomonitoring offers a simple and noninvasive approach for rapidly evaluating those exposures in real time. An electrochemical sensor coupled with a microflow injection system was developed for a simple, rapid, and sensitive characterization of cholinesterase (ChE) enzyme activities in rat saliva. The electrochemical sensor is based on a carbon nanotube (CNT)-modified screen-printed carbon electrode (SPE), which is integrated into a flow cell. Because of the excellent electrocatalytic activity of the CNTs, the sensor can detect electroactive species that are produced from enzymatic reactions with extremely high sensitivity and at low potentials. The electrochemical properties of acetylcholinesterase (AChE) enzymatic products were studied using a CNT-modified SPE, and the operation parameters such as the applied potential and substrate concentration were optimized to achieve the best performance. The AChE enzyme activity was further investigated using the CNT-based electrochemical sensor with commercially available purified AChE and ChE in saliva obtained from nave rats. It is found that the calibration curve is linear over a wide range of AChE concentrations from 5 pM to 0.5 nM, and the sensor is very sensitive with the detection limit down to 2 pM. The dynamics of the ChE enzyme activity in saliva with organophosphate pesticides was further studied using this sensor. The results show that the senor can be used to characterize salivary enzyme activity and to detect the exposure to organophosphate compounds. This new CNT-based electrochemical sensor thus provides a sensitive and quantitative tool for noninvasive biomonitoring of the exposure to organophosphate pesticides and nerve agents.
机译：某些唾液酶可能是有用的生物标记，可用于检测有机磷农药和化学神经毒剂的暴露。在这方面，唾液生物监测提供了一种简单且无创的方法，可以快速，实时地评估这些暴露。开发了一种与微流注射系统耦合的电化学传感器，用于简单，快速，灵敏地表征大鼠唾液中胆碱酯酶（ChE）酶的活性。电化学传感器基于碳纳米管（CNT）修饰的丝网印刷碳电极（SPE），该电极被集成到流通池中。由于CNT具有出色的电催化活性，因此该传感器可以以极高的灵敏度和低电位检测酶促反应产生的电活性物质。使用CNT修饰的SPE对乙酰胆碱酯酶（AChE）酶产物的电化学性质进行了研究，并优化了操作参数（如施加电势和底物浓度）以实现最佳性能。使用基于CNT的电化学传感器和从幼鼠获得的唾液中可商购的纯化AChE和ChE，进一步研究了AChE酶的活性。已发现，在从5 pM到0.5 nM的AChE浓度范围内，校准曲线是线性的，并且该传感器非常敏感，检测限低至2 pM。使用该传感器进一步研究了有机磷酸酯农药在唾液中ChE酶活性的动态。结果表明，该传感器可用于表征唾液酶活性并检测有机磷化合物的暴露。因此，这种新的基于CNT的电化学传感器为非侵入性生物监测有机磷酸酯农药和神经毒剂的暴露提供了灵敏且定量的工具。

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  • 来源
    《Environmental Science & Technology》 |2008年第7期|p.2688-2693|共6页
  • 作者

    JUN WANG; CHARLES TIMCHALK; YUEHE LIN;

  • 作者单位

    Pacific Northwest National Laboratory, Richland, Washington 99352;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
  • 关键词

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