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首页> 外文期刊>Environmental Science & Technology >Identification of Tylosin Photoreaction Products and Comparison off ELISA and HPLCMethods for Their Detection in Water
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Identification of Tylosin Photoreaction Products and Comparison off ELISA and HPLCMethods for Their Detection in Water

机译：泰乐菌素光反应产物的鉴定及ELISA和HPLC方法在水中的检测方法比较

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摘要

Tylosin is a widely used macrolide antibiotic for therapeutics and growth promotion in swine, beef cattle, and poultry production. Through various routes such as manure application, emission, inappropriate disposal, etc., tylosin enters the environment. The fate of tylosin in the environment is not yet fully understood. In this study, two photoreaction products of tylosin in water were identified as isotylosin A alcohol (E,Z) and isotylosin A aldol (E,Z). Tylosin A, B, C, D, isotylosin A alcohol, and isotylosin A aldol were purified, and immunological cross-reactivities of these tylosin-related compounds were tested with a specificity of 26% for tylosin B, 19% for tylosin C, 106% for tylosin D, 121% for isotylosin A alcohol, and 46% for isotylosin A aldol, compared to 100% for tylosin A. Competitive direct enzyme-linked immunosorbent assay (ELISA) for tylosin detection in water was compared with a high-performance liquid chromatography (HPLC) method by analyzing the same water samples from a study of tylosin dissipation in water. ELISA kits detect the other tylosin-related compounds besides tylosin A, which can result in differences in tylosin determination in water.
机译：泰乐菌素是一种广泛使用的大环内酯类抗生素，可用于猪，肉牛和家禽的治疗和生长促进。泰乐菌素通过各种方式（如施肥，排放，处置不当等）进入环境。泰乐菌素在环境中的命运尚未完全了解。在这项研究中，泰乐菌素在水中的两个光反应产物被鉴定为异麦芽酚素A醇（E，Z）和异麦芽酚素A醇醛（E，Z）。纯化了泰乐菌素A，B，C，D，异泰乐菌素A醇和异泰乐菌素A醇醛，并测试了这些与泰乐菌素相关的化合物的免疫交叉反应性，其中泰乐菌素B的特异性为26％，泰乐菌素C的特异性为19％，106泰乐菌素D为100％，异泰乐菌素A醇为121％，异泰乐菌素A醇为46％，泰乐菌素A为100％。竞争性直接酶联免疫吸附测定（ELISA）用于水中泰乐菌素检测与高性能液相色谱（HPLC）方法通过分析水中的泰乐菌素消散研究来分析相同的水样。 ELISA试剂盒可检测除泰乐菌素A以外的其他泰乐菌素相关化合物，这可能会导致水中泰乐菌素测定的差异。

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