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首页> 外文期刊>Environmental Science & Technology >Examination of Isomer Specific Bioaccumulation Parameters and Potential In vivo Hepatic Metabolites of syn- and anfi-Dechlorane Plus Isomers in Juvenile Rainbow Trout (Oncorhynchus mykiss)
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Examination of Isomer Specific Bioaccumulation Parameters and Potential In vivo Hepatic Metabolites of syn- and anfi-Dechlorane Plus Isomers in Juvenile Rainbow Trout (Oncorhynchus mykiss)

机译：幼体虹鳟鱼（Oncorhynchus mykiss）的异构体特异性生物富集参数和顺式和反式十氯烷加异构体的体内潜在肝代谢物检测

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Juvenile rainbow trout (Oncorhynchus mykiss) were exposed in the laboratory to elevated doses of syn- and anti-isomers of Dechlorane Plus (DP) via their diet for 49 days (uptake phase), followed by 112 days of untreated food (depuration phase) to examine bioaccumulation parameters and possible metabolic products. Three groups of 60 fish were used in the study. Two groups were exposed separately to food fortified with known concentrations of syn- (0.79 ± 0.03 μg/g, lipid weight) and anti-DP (1.17 ± 0.12 μg/g, lipid weight) while a third control group was fed unfortified food. Neither isomer reached steady-state after 49 days of exposure. Only the syn-isomer accumulated linearly in the fish (whole-body minus liver) during the dosing phase with a calculated uptake rate constant of 0.045 ± 0.005 (arithmetic mean ± 1 × standard error) nmoles per day. A similar uptake rate was also observed for this isomer in the liver. The elimination of both isomers from the whole fish (minus liver) obeyed first order depuration kinetics (syn-: r~2 = 0.6427, p < 0.001, anti-: r~2=0.5350, p < 0.005) with calculated half-lives (t_(1/2)) of 53.3 ± 13.1 (syn-) and 30.4 ± 5.7 (anti-) days. Elimination of the isomers from the liver was difficult to interpret because of suspected enterohepatic circulation and redistribution of the isomers in the liver during clearance from other tissues. The biomagnification factor (BMF, determined in whole fish minus liver) of the syn-isomer (5.2) was greater than the anti-isomer (1.9) suggesting that the former isomer is more bioavailable. A suite of metabolites were screened for in the liver including dechlorinated, hydroxylated, methoxylated and methyl sulfone degradates. Even with the purposely high dose used in the uptake phase, none of these degradates could be detected in the extracts. This suggests that if metabolites of DP are detected in fish from aquatic food webs their presence is likely not from in vivo biotransformation of the parent compound.
机译：幼体虹鳟鱼（Oncorhynchus mykiss）在实验室中通过饮食接触高剂量的Dechlorane Plus（DP）的同分异构体和反异构体，持续49天（摄取阶段），然后经过112天未经处理的食物（净化阶段）检查生物蓄积参数和可能的代谢产物。研究中使用了三组60条鱼。两组分别暴露于以已知浓度的syn-（0.79±0.03μg/ g，脂质重量）和抗DP（1.17±0.12μg/ g，脂质重量）强化的食品中，而第三组则喂养未经强化的食品。暴露49天后，两种异构体均未达到稳态。在给药阶段，仅顺式异构体在鱼（全身减去肝脏）中线性积累，计算的摄取速率常数为每天0.045±0.005（算术平均值±1×标准误差）nmol。在肝脏中也观察到该异构体的相似吸收率。从整个鱼类（减去肝脏）中消除两种异构体均符合一级净化动力学要求（syn-：r〜2 = 0.6427，p <0.001，anti-：r〜2 = 0.5350，p <0.005），具有半衰期（t_（1/2））为53.3±13.1（syn-）天和30.4±5.7（反）天。从肝脏中消除异构体很难解释，因为在从其他组织清除过程中怀疑存在肝肠循环和异构体在肝脏中的重新分布。同分异构体（5.2）的生物放大系数（BMF，在整条鱼中减去肝脏确定）大于反异构体（1.9），表明前者具有更高的生物利用度。在肝脏中筛选了一组代谢物，包括脱氯，羟化，甲氧基化和甲基砜降解物。即使在摄取阶段使用了故意的高剂量，提取物中也未检测到这些降解物。这表明，如果从水生食物网中的鱼中检测到DP的代谢产物，则它们的存在可能不是母体化合物的体内生物转化引起的。

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