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首页> 外文期刊>Environmental Science & Technology >Gene Expression Profiling in Common Cormorant Liver with an Oligo Array:Assessing the Potential Toxic Effects of Environmental Contaminants
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Gene Expression Profiling in Common Cormorant Liver with an Oligo Array:Assessing the Potential Toxic Effects of Environmental Contaminants

机译：利用寡核苷酸阵列在普通Live肝中进行基因表达谱分析：评估环境污染物的潜在毒性作用

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摘要

To establish a monitoring system for gene expression profiles related to chemical contamination in wild common cormorants (Phalacrocorax carbo),the present study constructed an oligo array designed from expressed sequence tag (EST) sequences of the cormorant liver,where 1061 unique oligonucleotides were spotted.Common cormorants were collected from Lake Biwa,Japan in May 2001 and 2002.With the use of this oligo array,gene expression profiles in the liver of individual specimens were evaluated.To determine the expression patterns of genes altered by environmental contaminants,relationships between concentrations of persistent organochlorines including polychlorinated dibenzo-p-dioxins,furans,polychlorinated biphenyls,1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane and its metabolites (DDTs),hexachlorocyclohexane isomers (HCHs),chlordane compounds (CHLs),butyltins,and bisphenol A (BPA) and expression levels of each gene in the cormorant liver were examined using stepwise multiple regression analysis.The reliability of data obtained by the oligo array was further confirmed by quantifying the expression levels of certain genes using real-time RT-PCR.The 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent (TEQ) level was positively correlated with both cytochrome P4501A4 and 1A5 gene expression.In addition,the mRNA level of an antioxidant enzyme,Cu/Zn superoxide dismutase,was negatively correlated with hepatic total TEQ.Other antioxidant enzymes,glutathione peroxidase 3 and glutathione S-transferase class mu,were negatively correlated with HCHs and BPA levels,respectively.The.mRNA expression level of a nonenzymatic antioxidant,haptoglobin,was negatively but not significantly correlated with CHLs.These results led to a hypothesis that wild cormorant population may suffer from oxidative stress due to chemically induced formation of reactive oxygen species and subsequent reduction of antioxidant resistance.Thus,the cormorant oligo array may be a useful monitoring tool to identify specific gene expression profiles altered by various environmental contaminants.Although further research is required to clarify a definitive cause-and-effect relationship,the current study provides valuable information on contaminant-responsive genes to predict potential effects on wildlife in a real environment.
机译：为了建立与野生普通cor（蝴蝶ala）化学污染相关的基因表达谱的监测系统，本研究构建了一种由array肝表达序列标签（EST）序列设计的寡核苷酸阵列，其中发现了1061个独特的寡核苷酸。分别于2001年5月和2002年5月从日本琵琶湖采集普通cor。使用该寡核苷酸阵列，评估单个标本肝脏中的基因表达谱。确定环境污染物改变的基因表达模式，浓度之间的关系多氯联苯，对二恶英，呋喃，多氯联苯，1,1,1-三氯-2,2-双（对氯苯基）乙烷及其代谢产物（DDT），六氯环己烷异构体（HCH），氯丹化合物的持久性有机氯（CHLs），丁基锡和双酚A（BPA）以及using肝中各基因的表达水平采用逐步多元回归分析n分析。通过实时RT-PCR定量某些基因的表达水平，进一步证实了寡核苷酸阵列获得的数据的可靠性.2,3,7,8-四氯二苯并-p-二恶英毒性当量（TEQ） P4501A4和1A5基因的表达与肝癌的发生呈正相关。此外，抗氧化酶Cu / Zn超氧化物歧化酶的mRNA水平与肝脏总TEQ呈负相关。非酶抗氧化剂触珠蛋白的mRNA表达水平与CHLs呈负相关，但与CHLs无显着相关。这些结果导致了一个假设，即野生population种群可能遭受氧化应激。由于化学诱导的活性氧的形成并随后降低了抗氧化性。因此，cor寡核苷酸阵列可能是一种有用的监测工具阐明受各种环境污染物改变的特定基因表达谱。尽管需要进一步研究以阐明确定的因果关系，但本研究提供了有关污染物响应基因的有价值的信息，以预测对真实环境中野生生物的潜在影响。

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  • 来源
    《Environmental Science & Technology》 |2006年第3期|p.1076-1083|共8页
  • 作者

    KEI NAKAYAMA; HISATO IWATA; EUN-YOUNG KIM; KOSUKE TASHIRO; SHINSUKE TANABE;

  • 作者单位

    Center for Marine Environmental Studies (CMES),Ehime University,Bunkyo-cho 2-5,Matsuyama 790-8577,Japan,and Faculty of Agriculture,Graduate School,Kyushu University,Hakozaki 6-10-1,Higashi-ku,Fukuoka 812-8581,Japan;

    Center for Marine Environmental Studies (CMES),Ehime University,Bunkyo-cho 2-5,Matsuyama 790-8577,Japan,and Faculty of Agriculture,Graduate School,Kyushu University,Hakozaki 6-10-1,Higashi-ku,Fukuoka 812-8581,Japan;

    Center for Marine Environmental Studies (CMES),Ehime University,Bunkyo-cho 2-5,Matsuyama 790-8577,Japan,and Faculty of Agriculture,Graduate School,Kyushu University,Hakozaki 6-10-1,Higashi-ku,Fukuoka 812-8581,Japan;

    Center for Marine Environmental Studies (CMES),Ehime University,Bunkyo-cho 2-5,Matsuyama 790-8577,Japan,and Faculty of Agriculture,Graduate School,Kyushu University,Hakozaki 6-10-1,Higashi-ku,Fukuoka 812-8581,Japan;

    Center for Marine Environmental Studies (CMES),Ehime University,Bunkyo-cho 2-5,Matsuyama 790-8577,Japan,and Faculty of Agriculture,Graduate School,Kyushu University,Hakozaki 6-10-1,Higashi-ku,Fukuoka 812-8581,Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
  • 关键词

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