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首页> 外文期刊>Environmental Science & Technology >Biological Validation of a Sample Preparation Method for ER-CALUX Bioanalysis of Estrogenic Activity in Sediment Using Mixtures of Xeno-Estrogens
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Biological Validation of a Sample Preparation Method for ER-CALUX Bioanalysis of Estrogenic Activity in Sediment Using Mixtures of Xeno-Estrogens

机译：异种雌激素混合物对沉积物雌激素活性进行ER-CALUX生物分析的样品制备方法的生物学验证

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摘要

The combined estrogenic effects of mixtures of environmental pollutants in the in vitro ER-CALUX (chemical activated luciferase gene expression) bioassay were examined to biologically validate a sample preparation method for the analysis of estrogenic compounds in sediment.The method used accelerated solvent extraction (ASE) and gel permeation chromatography (GPC) and was validated with respect to recovery of biological response taking mixture effects into account.Four mixtures of three to six xeno-estrogenic compounds (bisphenol A,4-nonylphenol,(4,4'-dichlorodiphenyl)trichloroethane,(2,4'-dichlorodiphenyl)-trichloroethane,dieldrin,'4-n-octylphenol,a-chlordane,dibutylphthalate,(4,4'-dichlorodiphenyl)dichloroethylene,and 2,4,5-trichlorobiphenyl) were prepared.Experimentally determined mixture effects were well described by the concept of concentration addition (CA),as expected for similarly acting compounds.Observed estradiol equivalence factors of the mixtures (on average 1.2+-0.3) agreed very well with the value predicted according to CA.The sample preparation method was then applied to pure mixtures of standards and to sediment spiked with one of the mixtures.Recoveries of estrogenic compounds were estimated by determination of their mixture potencies in ER-CALUX and compared to the mixture effects predicted by CA.Recoveries of estrogenic activity were between 80 and 129%,indicating that the additive behavior of mixtures of xeno-estrogens is well conserved during sample preparation.Together with an average repeatability of 18.3%,low average limit of detection (2.6+-1.8 pg of EEQ/ g),and coefficient of variance (3.5+-3.3%),this demonstrated the suitability of the sample preparation method for the analysis of mixtures of (xeno-)estrogenic compounds in sediment with the ER-CALUX assay.
机译：研究了环境污染物混合物在体外ER-CALUX（化学激活的荧光素酶基因表达）生物测定中的联合雌激素作用，以生物学方式验证了用于分析沉积物中雌激素化合物的样品制备方法。该方法采用了加速溶剂萃取（ASE） ）和凝胶渗透色谱法（GPC），并在考虑到混合效应的情况下验证了生物反应的恢复。三到六种异源雌激素化合物的四种混合物（双酚A，4-壬基酚，（4,4'-二氯二苯基）制备了三氯乙烷，（2,4'-二氯二苯基）-三氯乙烷，狄氏剂，'4-正辛基苯酚，α-氯丹，邻苯二甲酸二丁酯，（4,4'-二氯二苯基）二氯乙烯和2,4,5-三氯联苯。如对相似作用的化合物所期望的那样，通过浓度增加（CA）的概念很好地描述了实验确定的混合物效果。混合物的观察到的雌二醇当量因子（平均1.2 + -0.3）agr样品的制备方法适用于纯净标准混合物和掺有其中一种混合物的沉淀物，并通过测定其在ER-CALUX和HPLC中的混合力来估算其回收率。与CA预测的混合物效应相比，雌激素活性的回收率在80％至129％之间，这表明异种雌激素混合物的加成行为在样品制备过程中得到了很好的保留。加在一起的平均重复性为18.3％，平均下限低检测值（EEQ / g的2.6 + -1.8 pg）和变异系数（3.5 + -3.3％），这表明样品制备方法适用于分析沉积物中的（异）雌激素化合物的混合物ER-CALUX分析。

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  • 来源
    《Environmental Science & Technology》 |2006年第7期|p.2455-2461|共7页
  • 作者

    CORINE J.HOUTMAN; YONI K.VAN HOUTEN; PIM E.G.LEONARDS; ABRAHAM BROUWER; MARJA H.LAMOREE; JULIETTE LEGLER;

  • 作者单位

    Institute for Environmental Studies,Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,De Boelelaan 1087,1081 HV Amsterdam,The Netherlands,and Netherlands Institute for Fisheries Research, Haringkade 3,1970 AB Ymuiden,The Netherlands;

    Institute for Environmental Studies,Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,De Boelelaan 1087,1081 HV Amsterdam,The Netherlands,and Netherlands Institute for Fisheries Research, Haringkade 3,1970 AB Ymuiden,The Netherlands;

    Institute for Environmental Studies,Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,De Boelelaan 1087,1081 HV Amsterdam,The Netherlands,and Netherlands Institute for Fisheries Research, Haringkade 3,1970 AB Ymuiden,The Netherlands;

    Institute for Environmental Studies,Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,De Boelelaan 1087,1081 HV Amsterdam,The Netherlands,and Netherlands Institute for Fisheries Research, Haringkade 3,1970 AB Ymuiden,The Netherlands;

    Institute for Environmental Studies,Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,De Boelelaan 1087,1081 HV Amsterdam,The Netherlands,and Netherlands Institute for Fisheries Research, Haringkade 3,1970 AB Ymuiden,The Netherlands;

    Institute for Environmental Studies,Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam,De Boelelaan 1087,1081 HV Amsterdam,The Netherlands,and Netherlands Institute for Fisheries Research, Haringkade 3,1970 AB Ymuiden,The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
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