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首页> 外文期刊>Environmental Science & Technology >Monitoring Algal Toxins in Lake Water by Liquid Chromatography Tandem Mass Spectrometry
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Monitoring Algal Toxins in Lake Water by Liquid Chromatography Tandem Mass Spectrometry

机译：液相色谱-串联质谱法监测湖水中的藻毒素

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摘要

Microcystins (MCs)and cylindrospermopsin (CYL)are potent natural toxins produced by cyanobacteria (blue-green algae)that grow worldwide in eutrophic freshwaters and cause animal and human water-based toxicoses.The main purpose of this work has been assessing the contamination levels of some MCs and CYL in eutrophic Italian lake (Albano)water.To do this,we have developed an original analytical method involving MC extraction with a sorbent (Carbograph 4)cartridge.CYL is a highly polar compound that is scarcely retained by any sorbent material.To analyze this toxin,we directly injected 0.5 mL of filtered lake water into the liquid chromatography (LC)column.Analytes were quantified by LC coupled to tandem mass spectrometry in the multireaction monitoring mode.The recovery of five selected MCs added to an analyte-free lake water sample at three different concentrations (50,150,and 500 ng/L)ranged between 93 and 103% with RSD values no larger than 8%.Limits of quantification (LOQ)of the five MCs were within the 2-9 ng/L range,whereas the LOQ of CYL was 300 ng/L.The occurrence and abundance of cyanotoxins in Lake Albano was monitored over four months (Sept-Dec 2004)by analyzing water samples collected monthly at the center of the lake and at different depths (from 0 to -30 m).During survey and with the MS/MS system operating in the parent ion scan mode,we individuated two demethylated forms of MC-RR and one demethylated variety of MC-LR.Demethylated MC-RRs are known to be even more toxic than MC-RR toward zooplanktic grazers.CYL was the most-abundant toxin during the first three monitoring months.To the best of our knowledge,this is the first work reporting concentration levels of CYL in lake water.
机译：微囊藻毒素（cycystdrospermopsin，微囊藻毒素）和微囊藻毒素（cylindrospermopsin，CYL）是蓝藻（蓝绿藻）产生的强效天然毒素，它们在全球富营养化的淡水中生长，并引起动物和人类水基有毒物质。这项工作的主要目的是评估污染水平富营养性意大利湖泊（阿尔巴诺）水中的某些MC和CYL。为此，我们开发了一种原始方法，涉及用吸附剂（碳谱仪4）盒进行MC萃取。CYL是一种高极性化合物，几乎没有任何吸附剂保留为了对该毒素进行分析，我们将0.5 mL滤过的湖水直接注入液相色谱（LC）色谱柱中。在多反应监测模式下，通过LC与串联质谱联用对分析物进行定量。回收了5种选定的MC三种不同浓度（50,150和500 ng / L）的无分析物的湖水样品的浓度在93％至103％之间，RSD值不大于8％。这五种样品的定量限（LOQ） MCs在2-9 ng / L范围内，而CYL的LOQ为300 ng / L。通过分析每月在2004年9月至2004年12月收集的水样，监测了阿尔巴诺湖中氰毒素的发生和丰度。在调查中以及在MS / MS系统以母离子扫描模式运行的情况下，我们区分了MC-RR的两种脱甲基形式和MC的一种脱甲基形式-LR。去甲基化的MC-RRs对动物浮游动物的毒性甚至比MC-RR更高。CYL是头三个监测月中最丰富的毒素。据我们所知，这是第一个报告浓度的工作湖水中的CYL含量。

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  • 来源
    《Environmental Science & Technology》 |2006年第9期|p.2917-2923|共7页
  • 作者

    SARA BOGIALLI; MILENA BRUNO; ROBERTA CURINI; ANTONIO DI CORCIA; CHIARA FANALI;

  • 作者单位

    Dipartimento di Chimica,Universita La Sapienza,Piazza Aldo Mow 5,00185 Rome,Italy,and Dipartimento d'Igiene Ambientale,Istituto Nazionale di Sanita,Viale Regina Elena 299,00161 Rome,Italy;

    Dipartimento di Chimica,Universita La Sapienza,Piazza Aldo Mow 5,00185 Rome,Italy,and Dipartimento d'Igiene Ambientale,Istituto Nazionale di Sanita,Viale Regina Elena 299,00161 Rome,Italy;

    Dipartimento di Chimica,Universita La Sapienza,Piazza Aldo Mow 5,00185 Rome,Italy,and Dipartimento d'Igiene Ambientale,Istituto Nazionale di Sanita,Viale Regina Elena 299,00161 Rome,Italy;

    Dipartimento di Chimica,Universita La Sapienza,Piazza Aldo Mow 5,00185 Rome,Italy,and Dipartimento d'Igiene Ambientale,Istituto Nazionale di Sanita,Viale Regina Elena 299,00161 Rome,Italy;

    Dipartimento di Chimica,Universita La Sapienza,Piazza Aldo Mow 5,00185 Rome,Italy,and Dipartimento d'Igiene Ambientale,Istituto Nazionale di Sanita,Viale Regina Elena 299,00161 Rome,Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
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