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首页> 外文期刊>Environmental Science & Technology >Electrochemical detection and control of interactions between DNA and electroactive intercalator using a DNA - Alginate complex film modified electrode
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Electrochemical detection and control of interactions between DNA and electroactive intercalator using a DNA - Alginate complex film modified electrode

机译：DNA-海藻酸盐复合膜修饰电极对DNA与电活性嵌入剂之间相互作用的电化学检测和控制。

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摘要

The surface of a glassy carbon electrode was modified with a DNA-alginate complex film in which water-soluble DNA was encapsulated into a calcium- alginate gel. The resulting modified electrode (DAFE) was used to detect ethidium bromide (EtBr), after its accumulation on the electrode. The intercalative behavior of EtBr for dsDNA in the film was investigated by measuring the electrode response based on the intercalation of EtBr separated from nonspecific interactions (electrostatic interaction). The accumulation of EtBr in the dsDAF was enhanced by applying a negative potential below -200 mV at the dsDAFE. When a positive potential above +700 mV was applied to the dsDAFE for a constant time with stirring in a Tris buffer solution, the amount accumulated decreased. These results indicate that it is possible to electrochemically control the accumulation and release of EtBr when a dsDAFE is used. In addition, the accumulation and detection of EtBr in spiked river water samples and daunomycin, an antitumor agent, is described.
机译：玻碳电极的表面用DNA-海藻酸盐复合膜修饰，其中水溶性DNA封装在海藻酸钙凝胶中。所得修饰电极（DAFE）在其在电极上累积后，可用于检测溴化乙锭（EtBr）。通过基于与非特异性相互作用（静电相互作用）分离的EtBr的嵌入，通过测量电极响应来研究EtBr对dsDNA的嵌入行为。通过在dsDAFE上施加低于-200 mV的负电势，可以增强dsDAF中EtBr的积累。当在Tris缓冲溶液中搅拌的同时，将恒定+700 mV以上的正电势施加到dsDAFE时，累积的量减少。这些结果表明，当使用dsDAFE时，可以电化学控制EtBr的积累和释放。另外，还描述了加标河水样品和道诺霉素（一种抗肿瘤药）中EtBr的积累和检测。

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