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首页> 外文期刊>Russian journal of electrochemistry >Electrochemical DNA impedance biosensor for the detection of DNA hybridization with polymeric film, single walled carbon nanotubes modified glassy carbon electrode
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Electrochemical DNA impedance biosensor for the detection of DNA hybridization with polymeric film, single walled carbon nanotubes modified glassy carbon electrode

机译：电化学DNA阻抗生物传感器，用于检测聚合物膜，单壁碳纳米管修饰的玻碳电极与DNA的杂交

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摘要

In this work, we report the fabrication of a sensitive electrochemical DNA impedance biosensor for the detection of sequence-specific target DNA. p-Aminobenzoic acid was first immobilized on the surface of the electrode modified with single walled carbon nanotubes with carboxylic acid groups (SWCNTs) by cyclic voltammetry (CV). A single-stranded DNA probe with a NH_2 group at the end (H_2N-ssDNA) was then covalently immobilized on the surface of polymeric film at room temperature. The impedance measurement was performed in a solution containing 5 mM K_3[Fe(CN)_6]/K_4[Fe(CN)_6]. The change of interfacial charge transfer resistance (R_(CT)) was confirmed the hybrid formation. The difference of R_(CT) was linear with the logarithm of complementary oligonucleotides concentrations in the range of 1.0 × 10~(-12) to 1.0 × 10~(-7) M, with a detection limit of 3.5 × 10~(-13) M (S/N = 3).
机译：在这项工作中，我们报告了一种敏感的电化学DNA阻抗生物传感器的制造，用于检测序列特异性靶DNA。首先通过循环伏安法（CV）将对氨基苯甲酸固定在用具有羧酸基团的单壁碳纳米管（SWCNT）修饰的电极表面上。然后在室温下将末端具有NH_2基团的单链DNA探针（H_2N-ssDNA）共价固定在聚合物膜的表面上。在包含5 mM K_3 [Fe（CN）_6] / K_4 [Fe（CN）_6]的溶液中进行阻抗测量。界面电荷转移电阻（R_（CT））的变化证实了杂化的形成。 R_（CT）的差异与互补寡核苷酸浓度在1.0×10〜（-12）至1.0×10〜（-7）M的对数呈线性关系，检出限为3.5×10〜（- 13）M（S / N = 3）。

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