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首页> 外文期刊>Environmental Science & Technology >Evaluation of a novel malathion immunoassay for groundwater and surface water analysis
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Evaluation of a novel malathion immunoassay for groundwater and surface water analysis

机译：用于地下水和地表水分析的新型马拉硫磷免疫分析方法的评估

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摘要

Malathion is an organophosphorus insecticide commonly used in crops and indoor applications. Negative effects of malathion on human health and ecosystems are of growing concern. In this work, novel malathion haptens are synthesized to develop an ELISA screening method. The immunoassay is based on a conjugate-coated format and shows a limit of detection of 0.11 ng/mL, an IC50 of 1.58 ng/mL, a dynamic range between 0.23 and 10.94 ng/mL, and a cross-reactivity of < 2% with structurally related compounds. The developed ELISA has been used to quantify malathion in groundwater and surface water samples. The good recoveries achieved and the agreement with those given by the GC-MS reference method indicate the potential of the assay for environmental monitoring of malathion in natural waters without purification or preconcentration steps. The effect of dissolved organic matter (humic acids) on the ELISA was evaluated, resulting in the conclusion that the immunoassay can be successfully performed in surface water samples with a humic acid content up to 10 mg/L, without sample pretreatment. Samples with a high humic acid content can be analyzed through ELISA after solid-phase extraction, eluting with acetone or acetonitrile.
机译：马拉硫磷是一种有机磷杀虫剂，通常用于农作物和室内应用。马拉硫磷对人类健康和生态系统的负面影响日益引起人们的关注。在这项工作中，新型马拉硫磷半抗原被合成以开发ELISA筛选方法。免疫测定是基于结合物包被的形式，检测限为0.11 ng / mL，IC50为1.58 ng / mL，动态范围为0.23至10.94 ng / mL，交叉反应率<2％与结构相关的化合物。发达的ELISA已用于定量地下水和地表水样品中的马拉硫磷。所获得的良好回收率以及与GC-MS参考方法所给出的回收率相吻合，表明该方法可用于天然水中马拉硫磷的环境监测而无需纯化或预浓缩步骤的潜力。评估了溶解的有机物（腐殖酸）对ELISA的影响，得出的结论是，无需进行样品预处理，就可以成功地对腐殖酸含量高达10 mg / L的地表水样品进行免疫测定。固相萃取后，可通过ELISA分析高腐殖酸含量的样品，并用丙酮或乙腈洗脱。

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