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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Evaluation of Murine Norovirus Feline Calicivirus Poliovirus and MS2 as Surrogates for Human Norovirus in a Model of Viral Persistence in Surface Water and Groundwater
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Evaluation of Murine Norovirus Feline Calicivirus Poliovirus and MS2 as Surrogates for Human Norovirus in a Model of Viral Persistence in Surface Water and Groundwater

机译：鼠诺如病毒猫杯状病毒脊髓灰质炎病毒和MS2作为人类诺如病毒的替代品在地表水和地下水中的病毒持久性模型中的评估。

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Human noroviruses (NoVs) are a significant cause of nonbacterial gastroenteritis worldwide, with contaminated drinking water a potential transmission route. The absence of a cell culture infectivity model for NoV necessitates the use of molecular methods and/or viral surrogate models amenable to cell culture to predict NoV inactivation. The NoV surrogates murine NoV (MNV), feline calicivirus (FCV), poliovirus (PV), and male-specific coliphage MS2, in conjunction with Norwalk virus (NV), were spiked into surface water samples (n = 9) and groundwater samples (n = 6). Viral persistence was monitored at 25°C and 4°C by periodically analyzing virus infectivity (for all surrogate viruses) and nucleic acid (NA) for all tested viruses. FCV infectivity reduction rates were significantly higher than those of the other surrogate viruses. Infectivity reduction rates were significantly higher than NA reduction rates at 25°C (0.18 and 0.09 log10/day for FCV, 0.13 and 0.10 log10/day for PV, 0.12 and 0.06 log10/day for MS2, and 0.09 and 0.05 log10/day for MNV) but not significant at 4°C. According to a multiple linear regression model, the NV NA reduction rates (0.04 ± 0.01 log10/day) were not significantly different from the NA reduction rates of MS2 (0.05 ± 0.03 log10/day) and MNV (0.04 ± 0.03 log10/day) and were significantly different from those of FCV (0.08 ± 0.03 log10/day) and PV (0.09 ± 0.03 log10/day) at 25°C. In conclusion, MNV shows great promise as a human NoV surrogate due to its genetic similarity and environmental stability. FCV was much less stable and thus questionable as an adequate surrogate for human NoVs in surface water and groundwater.
机译：人类诺如病毒（NoVs）是全世界非细菌性胃肠炎的重要原因，饮用水污染是潜在的传播途径。没有针对NoV的细胞培养物感染性模型，必须使用适合于细胞培养来预测NoV灭活的分子方法和/或病毒替代模型。将NoV替代鼠NoV（MNV），猫杯状病毒（FCV），脊髓灰质炎病毒（PV）和雄性特异性噬菌体MS2与Norwalk病毒（NV）一起掺入地表水样品（n = 9）和地下水样品中（n = 6）。通过定期分析病毒感染性（对于所有替代病毒）和核酸（NA）对所有测试病毒的监测，分别在25°C和4°C下监测病毒的持久性。 FCV传染性降低率显着高于其他替代病毒。在25°C时，传染性降低率显着高于NA降低率（FCV为0.18和0.09 log10 /天，PV为0.13和0.10 log10 /天，MS2为0.12和0.06 log10 /天，MS2为0.09和0.05 log10 /天MNV），但在4°C下不显着。根据多元线性回归模型，NV NA减少率（0.04±0.01 log10 /天）与MS2（0.05±0.03 log10 /天）和MNV（0.04±0.03 log10 /天）的NA减少率没有显着差异。并且在25°C下与FCV（0.08±0.03 log10 /天）和PV（0.09±0.03 log10 /天）显着不同。总之，由于其遗传相似性和环境稳定性，MNV作为人类NoV替代品具有广阔的前景。 FCV的稳定性要差得多，因此不能作为地表水和地下水中人类NoV的适当替代品。

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