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首页> 外文期刊>Environmental Science & Technology >Chemically activated luciferase gene expression (CALUX) cell bioassay analysis for the estimation of dioxin-like activity: Critical parameters of the CALUX procedure that impact assay results
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Chemically activated luciferase gene expression (CALUX) cell bioassay analysis for the estimation of dioxin-like activity: Critical parameters of the CALUX procedure that impact assay results

机译：化学活化的萤光素酶基因表达（CALUX）细胞生物测定分析，以评估二恶英样活性：影响测定结果的CALUX程序的关键参数

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摘要

The chemically activated luciferase gene expression (CALUX) in vitro cell bioassay is a bioanalytical tool that is increasingly being used by research and commercial laboratories for the screening and relative quantification of dioxins and dioxin-like compounds in sample extracts. Since CALUX analyses provide a biological response to all aryl hydrocarbon receptor active compounds present in a given sample extract containing a complex mixture of chemicals, interpretation of results is significantly more complex than of chemical analyses. Operators in the laboratory can adjust many parameters when performing CALUX analyses, and the applied procedure strongly affects the result and, hence, the interpretation of the results. This paper examines critical methodological parameters and aspects of the CALUX bioassay that can affect the quality and accuracy of the analyses. Moreover, the study aims to identify the ways that a Iteration of these parameters influences CALUX measurements. A greater understanding of these characteristics will lead to increased accuracy, precision, and reproducibility of the widely used CALUX bioassay within and between research laboratories.
机译：化学激活的萤光素酶基因表达（CALUX）体外细胞生物测定是一种生物分析工具，研究和商业实验室越来越多地使用它来筛选和定量分析样品提取物中的二恶英和二恶英样化合物。由于CALUX分析可对包含复杂化学混合物的给定样品提取物中存在的所有芳基烃受体活性化合物提供生物学响应，因此结果的解释要比化学分析复杂得多。进行CALUX分析时，实验室的操作员可以调整许多参数，并且所应用的程序会严重影响结果，并因此影响结果的解释。本文研究了可能影响分析质量和准确性的CALUX生物测定的关键方法学参数和方面。此外，该研究旨在确定这些参数的迭代影响CALUX测量的方式。对这些特征的更深入了解将导致研究实验室内部和研究实验室之间广泛使用的CALUX生物测定的准确性，准确性和可重复性的提高。

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