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Biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membrane

机译:从反渗透膜回收的细菌分离物的生物膜形成特征

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High-quality water purification systems using reverse osmosis (RO) membrane separation have faced a major challenge related to biofilm formation on the membrane surface, or biofouling. To understand this issue, the biofilm formation characteristics of four bacterial isolates previously retrieved from an RO membrane treating potable water were investigated. Biofilm formation of all four isolates occurred to different extents in microtiter plates and could be related to one or more cell properties (hydrophobicity, surface charge, and motility). For Dermacoccus sp. strain RO12 and Microbacterium sp. strain RO18, bacterial adhesion was facilitated by cell surface hydrophobicity, and for Rhodopseudomonas sp. strain RO3, adhesion was assisted by its low surface charge. Sphingomonas sp. strain RO2 possessed both twitching and swarming motilities, which could be importantin mediating surface colonization. Further, strains RO2, RO3, and RO12 did not exhibit swimming motility, suggesting that they could be transported to RO membrane surfaces by other mechanisms such as convective permeate flow. The biofilm formation of RO2 was further tested on different RO membranes made of cellulose acetate, polyamide, and thin film composite in continuous flow cell systems. The resultant RO2 biofilms were independent of membrane surface properties and this was probably related to the exopolysaccharides secreted by the biofilm cells. These results suggested that RO2 could colonize RO membranes effectively and could be a potential fouling organism in RO membranes for freshwater purification.
机译:使用反渗透(RO)膜分离技术的高质量水净化系统面临着与膜表面生物膜形成或生物污染相关的重大挑战。为了理解这个问题,对先前从处理饮用水的反渗透膜中回收的四种细菌分离物的生物膜形成特性进行了研究。所有四种分离物的生物膜形成都在微量滴定板中以不同程度发生,并且可能与一种或多种细胞特性(疏水性,表面电荷和运动性)有关。对于Dermacoccus sp。 RO12菌株和Microbacterium sp。菌株RO18,细菌粘附是由细胞表面疏水性促进的,对于Rhodopseudomonas sp。菌株RO3,其低表面电荷有助于粘附。鞘氨醇单胞菌RO2菌株同时具有抽动和成簇的功能,这在介导表面定殖中可能很重要。此外,菌株RO2,RO3和RO12没有表现出游动性,表明它们可以通过其他机制(例如对流渗透流)运输到RO膜表面。在连续流动池系统中,在由醋酸纤维素,聚酰胺和薄膜复合材料制成的不同RO膜上进一步测试了RO2的生物膜形成。最终的RO2生物膜与膜表面特性无关,这可能与生物膜细胞分泌的胞外多糖有关。这些结果表明,RO2可以有效地定殖在RO膜上,并且可能是RO膜中潜在的污垢生物,可以用于淡水净化。

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