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首页> 外文期刊>Environmental Science & Technology >Cadmium Induction of Metallothionein Isoforms in Juvenile and Adult Mussel (Mytilus edulis)
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Cadmium Induction of Metallothionein Isoforms in Juvenile and Adult Mussel (Mytilus edulis)

机译：少年和成年贻贝（Mytilus edulis）中金属硫蛋白亚型的镉诱导

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摘要

Isoforms of metallothionein in the digestive gland of control and experimentally Cd-exposed mussels (Mytilus edulis) (200μg L{sup}(-1) Cd{sup}(2+) and 400μg L{sup}(-1) Cd{sup}(2+); 20 days) were studied using the reverse transcriptase-polymerase chain reaction. In addition, glutathione S-transferase (GSTpi) primers were designed to evaluate the reduction in the antioxidant defense systems (glutathione) accompanying the aging process in the same organisms. Following experimental exposure, an accumulation of Cd was observed in the digestive gland of exposed mussels, both adults and juveniles, up to 500 times higher than in the control. An induction of the dimeric form MT20 II was detected in 400 μg L{sup}(-1) exposed mussels, as well as a visible inhibition of the monomeric form MT10 IV. After 20 days of exposure juveniles expressed increased GSTpi compared with adults. Results reveal individual variation of both metallothioneins and GSTpi expression among control and Cd{sup}(2+)-exposed mussels of different ages. The ecotoxicological significance of MT utilization in biomonitoring of seawater for trace metals has been considered in light of these results.
机译：对照和实验性Cd贻贝（Mytilus edulis）（200μgL {sup}（-1）Cd {sup}（2+）和400μgL {sup}（-1）Cd {sup）的消化腺中金属硫蛋白的同工型}（2+）; 20天）使用逆转录酶-聚合酶链反应进行了研究。此外，还设计了谷胱甘肽S-转移酶（GSTpi）引物，以评估同一生物中伴随老化过程而产生的抗氧化防御系统（谷胱甘肽）的减少。实验暴露后，在成年贻贝和成年贻贝的裸露贻贝的消化腺中发现了Cd积累，比对照高500倍。在暴露于400μgL {sup}（-1）的贻贝中检测到二聚体形式MT20 II的诱导，以及对单体形式MT10 IV的可见抑制。与成年人相比，暴露20天后，青少年表达的GSTpi升高。结果显示，不同年龄的对照组和Cd {sup}（2+）暴露的贻贝之间金属硫蛋白和GSTpi表达的个体差异。根据这些结果，已经考虑了MT在海水中微量金属生物监测中的生态毒理学意义。

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