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TCE Remediation Using In Situ, Resting-State Rioaugmentation

机译：使用原位静止状态增强术的TCE修复

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A field test has demonstrated that an in situ biofilter using resting-state cells effectively remediated groundwater with about 425 ppb of trichloroethene (TCE) as the sole contaminant species. About 5.4 kg (dry weight equivalent) of a strain of methanotrophic bacteria [Methylosinus trichosporium OB3b) was suspended in 1800 L of groundwater (5.4 x 10~9 cells/ mL) and injected into an aquifer through a single well at a depth of 27 m, several meters below the water table. The injected groundwater was devoid of TGE and growth substrates but was amended with a phosphate solution (10 mM) to buffer the pH and phenol red (20 μm) to act as a tracer. Approximately 50% of the injected bacteria attached to the sediments, forming an in situ, fixed-bed bioreactor of unknown geometry. Contaminated groundwater was subsequently withdrawn through the biofilter region by extracting at 3.8 L/min for 30 h and then at 2.0 L/min for the remaining 39 days of the field experiment. TCE concentrations in the extracted groundwater decreased from 425 to less than 10 ppb during the first 50 h of withdrawal, which is equivalent to a 98% reduction. TCE concentration extracted through the biofilter gradually increased to background values at 40 days when the experiment was terminated.
机译：现场测试表明，使用静止状态细胞的原位生物滤池可有效修复地下水，其中约425 ppb的三氯乙烯（TCE）是唯一的污染物。将约5.4千克（干重当量）的甲烷氧化营养菌[Methylosinus trichosporium OB3b]悬浮在1800 L地下水（5.4 x 10〜9个细胞/ mL）中，并通过一个单井注入深水层，深度为27米，在地下水位以下几米处。注入的地下水不含TGE和生长底物，但用磷酸盐溶液（10 mM）进行了修改，以缓冲pH值和酚红（20μm），用作示踪剂。大约50％的注射细菌附着在沉积物上，形成了未知几何形状的原位固定床生物反应器。随后，通过以3.8 L / min的速度提取30小时，然后以2.0 L / min的速度提取剩余的39天的田间实验，将污染的地下水通过生物滤池区域抽出。在撤出的最初50小时内，提取的地下水中三氯乙烯的浓度从425降至10 ppb以下，相当于降低了98％。当实验终止时，通过生物滤池提取的TCE浓度在40天时逐渐增加到背景值。

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