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首页> 外文期刊>Environmental Technology >Isolation of active coagulant protein from the seeds of Strychnos potatorum - a potential water treatment agent
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Isolation of active coagulant protein from the seeds of Strychnos potatorum - a potential water treatment agent

机译：从马铃薯马兜铃种子中分离出活性凝血蛋白-一种潜在的水处理剂

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The application of natural coagulants for decentralized water treatment is gaining importance as a part of global sustainable initiative. This study focuses on the isolation of active coagulation components responsible for water clarification with respect to Strychnos potatorum seeds. The active coagulant components, protein and polysaccharide, were successfully isolated, dialysed and subjected to gel permeation chromatography. The polysaccharide was isolated with the saline extraction method and characterized using FTIR and NMR spectroscopy. Protein was precipitated with 80% ammonium sulphate solution combined with dialysis and gel permeation chromatography using Sephadex G-50. SDS-PAGE revealed that the isolated protein has a molecular weight of 12 kDa. The small-scale coagulation assay suggests that the protein fraction has superior coagulation activity than the isolated polysaccharide residue. The active coagulant fractions reported in this study would be helpful in deploying cheaper and simple methods in scaling up the coagulant fraction from these seeds acting as a potential water treatment agent.
机译：作为全球可持续计划的一部分，将天然凝结剂用于分散水处理的重要性日益提高。这项研究的重点是分离对马兜铃种子进行水澄清的活性凝结成分。成功分离了活性凝结剂成分，蛋白质和多糖，进行了透析，并进行了凝胶渗透色谱分析。用盐水提取法分离多糖，并用FTIR和NMR光谱表征。用80％硫酸铵溶液结合透析和凝胶渗透色谱法（使用Sephadex G-50）沉淀蛋白质。 SDS-PAGE显示分离的蛋白质具有12kDa的分子量。小规模的凝血测定表明该蛋白质级分具有比分离的多糖残基更高的凝血活性。这项研究中报道的活性凝结剂级分将有助于部署更便宜，更简单的方法来放大这些种子中的凝结剂级分，从而起到潜在的水处理剂的作用。

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