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首页> 外文期刊>Environmental toxicology and pharmacology >Metabolomics study of cadmium-induced diabetic nephropathy and protective effect of caffeic acid phenethyl ester using UPLC-Q-TOF-MS combined with pattern recognition
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Metabolomics study of cadmium-induced diabetic nephropathy and protective effect of caffeic acid phenethyl ester using UPLC-Q-TOF-MS combined with pattern recognition

机译：UPLC-Q-TOF-MS结合模式识别技术研究镉诱导的糖尿病肾病的代谢组学和咖啡酸苯乙酯的保护作用

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摘要

Diabetic nephropathy (DN) is the most severe complication of diabetes and multiple factors are involved in the pathogenesis of DN. Among them, cadmium (Cd) acts as a risk factor inducing the occurrence of DN. The present study focused on investigating the protective role of caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, against Cd-induced DN in mice based on ultra performance liquid chromato-graphy coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS)and pattern recognition. Serum and urine biochemical indexes were detected and histopathological observation has been done to evaluate the damage of Cd on animals. Moreover, the global serum profiles of different groups were distinguished by UPLC-Q-TOF-MS and principal component analysis (PCA) were applied for group differentiation and marker selection. Moreover, the influence of Cd on the oxidative status in DN mice were also evaluated by assessing the parameters of oxidative stress, proinflammatory cytokines and antioxidant competence. As shown in the scores plots, the distinct clustering among controls, DN and CAPE groups were observed, significant changes in serum levels of LysoPC(18:l(llZ)), 2,3-dinor-8-iso-PGF2a, PS(18:1(9Z)/18:1(9Z)), DG(17:0/22:4 (7Z.10Z, 13Z, 16Z)/ 0:0) and Arachidonic acid(AA) were noted and identified as potential biomarkers, the effect of CAPE reverted them back to near normalcy. Further, It was observed a significant improvement in lipid peroxides (LPO) and protein carbonyls (PCO) levels in Cd-induced DN kidneys along with a significant decline in superoxide dis-mutase (SOD), catalase (CAT), and reduced glutathione (GSH) levels, however, CAPE relieved these changes. In conclusion, the study suggested that the pathogenesis of DN caused by Cd probably owes to the perturbations of lipid metabolism and AA metabolism; CAPE seems to be effective agent and may be related to its potent antioxidant, anti-inflammatory properties and action as an Nrf2 activator.
机译：糖尿病肾病（DN）是糖尿病最严重的并发症，并且DN的发病机制涉及多种因素。其中，镉（Cd）是引起DN发生的危险因素。基于超高效液相色谱结合四极杆飞行时间，本研究致力于研究蜜蜂蜂巢蜂胶中的咖啡酸苯乙酯（CAPE）对镉诱导的DN的保护作用。质谱（UPLC-Q-TOF-MS）和模式识别。检测血清和尿液的生化指标，并进行组织病理学观察以评估镉对动物的伤害。此外，通过UPLC-Q-TOF-MS区分了不同组的总体血清谱，并将主成分分析（PCA）用于组分化和标记选择。此外，还通过评估氧化应激，促炎细胞因子和抗氧化剂能力等参数来评估镉对DN小鼠氧化状态的影响。如得分图所示，在对照组，DN和CAPE组之间观察到明显的聚类，LysoPC（18：l（llZ）），2,3-dinor-8-iso-PGF2a，PS（注意到18：1（9Z）/ 18：1（9Z）），DG（17：0/22：4（7Z.10Z，13Z，16Z）/ 0：0）和花生四烯酸（AA）被确定为潜在生物标志物，CAPE的作用使它们恢复到接近常态。此外，还发现在Cd诱导的DN肾脏中，脂质过氧化物（LPO）和蛋白质羰基（PCO）含量显着提高，而超氧化物歧化酶（SOD），过氧化氢酶（CAT）显着下降，并且谷胱甘肽减少（但是，CAPE缓解了这些变化。总之，研究表明，镉引起的DN的发病机制可能归因于脂质代谢和AA代谢的扰动。 CAPE似乎是有效的药物，可能与其有效的抗氧化剂，抗炎特性以及作为Nrf2激活剂的作用有关。

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  • 来源
    《Environmental toxicology and pharmacology》 |2017年第9期|80-92|共13页
  • 作者

    Pin Gong; Xiangna Chang; Xuefeng Chen; Xiaohuan Bai; He Wen; Sihui Pi; Wenjuan Yang; Lan Wang; Fuxin Chen;

  • 作者单位

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    College of Food and Biotechnology, Shaanxi University of Science and Technology, Xi'an, 710021, China;

    School of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an, 710054, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Diabetic nephropathy; Caffeic acid phenethyl ester; Cadmium; UPLC-Q-TOF-MS;

    机译：糖尿病肾病;咖啡酸苯乙酯;镉;UPLC-Q-TOF-MS;

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