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首页> 外文期刊>Environmental toxicology and chemistry >SEDIMENT TOXICITY IDENTIFICATION EVALUATION (TIE PHASES I AND II) BASED ON MICROSCALE BIOASSAYS FOR DIAGNOSING CAUSES OF TOXICITY IN COASTAL AREAS AFFECTED BY DOMESTIC SEWAGE
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SEDIMENT TOXICITY IDENTIFICATION EVALUATION (TIE PHASES I AND II) BASED ON MICROSCALE BIOASSAYS FOR DIAGNOSING CAUSES OF TOXICITY IN COASTAL AREAS AFFECTED BY DOMESTIC SEWAGE

机译：基于微尺度生物分析的沉积物毒性鉴定（诊断阶段I和II），用于诊断生活污水对沿海地区的毒性

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Domestic sewage is a major problem in highly urbanized coastal areas worldwide. In the present study, toxicity identification evaluation (TIE) phases I and II were applied to sediment interstitial water from 2 locations along the Sao Paulo coast in southeastern Brazil: the sewage outfalls from the city of Santos, a densely urbanized area, and the city of Bertioga, a less urbanized area. An adapted microscale sea urchin embryo-larval development bioassay was employed. Phase TIE-I manipulations were 1) ethylenediamine tetraacetic acid (EDTA) addition, 2) aeration at modified pH, 3) C18 solid-phase extraction (SPE), and 4) addition of Na2S2O3. The results of the Santos TIE-I indicated toxicity by sulfides and substances with affinity to C18 resin. In Bertioga, toxicity was the result of NH3, metals, and arsenic, as well as substances with affinity to C18 resin. Phase TIE-II aimed to specify the causes of toxicity by testing the toxicity of eluates of the sublation and C18 SPE manipulation. The results reinforced the role of both surfactants and nonpolar compounds as causative agents of toxicity in both Santos and Bertioga. Chemical analyses of sediment interstitial water or whole sediment ruled out the influence of polycyclic aromatic hydrocarbons (PAHs) or polychlorinated biphenyls (PCBs) in the toxicity of both sampling sites. Other hydrophobic substances may play a role in the toxicity of Santos and Bertioga effluents. Efforts to remove such substances from sanitary effluents must be prioritized. (C) 2017 SETAC
机译：在世界上高度城市化的沿海地区，生活污水是一个主要问题。在本研究中，毒性鉴定评估（TIE）的阶段I和II被应用于巴西东南部圣保罗海岸两处的沉积物填隙水：来自桑托斯市，城市密集地区和城市的污水排放口Bertioga的城市化程度较低的地区。采用了适合的微型海胆胚胎-幼虫发育生物测定法。 TIE-1阶段的操作是：1）加入乙二胺四乙酸（EDTA），2）在改良的pH下通气，3）C18固相萃取（SPE）和4）添加Na2S2O3。 Santos TIE-1的结果表明了硫化物和对C18树脂具有亲和力的物质的毒性。在Bertioga中，毒性是NH3，金属和砷以及对C18树脂具有亲和力的物质的结果。 TIE-II期旨在通过测试沉浮洗脱液的毒性和C18 SPE操作来确定毒性的原因。该结果加强了表面活性剂和非极性化合物在桑托斯和贝蒂奥加中作为毒性的致病剂的作用。对沉积物间隙水或整个沉积物的化学分析排除了多环芳烃（PAHs）或多氯联苯（PCBs）对两个采样点的毒性的影响。其他疏水性物质可能在Santos和Bertioga废水的毒性中起作用。必须优先考虑从卫生废水中去除此类物质的努力。 （C）2017年SETAC

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